Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2023 Dec 23

PMID 38138386

Authors

Elena N Esimbekova

Irina G Torgashina

Elena V Nemtseva

Valentina A Kratasyuk

Affiliations

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Abstract

The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in biotechnology for producing enzyme preparations stable during use and storage and insensitive to chemical and physical impacts. The authors summarize their achievements in developing the preparations of enzymes immobilized in starch and gelatin gels and assess their activity, stability, and sensitivity for use as biorecognition elements of enzyme inhibition-based biosensors.

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References

French D . Chemical and physical properties of starch. J Anim Sci. 1973; 37(4):1048-61. DOI: 10.2527/jas1973.3741048x. View

Esimbekova E, Kirillova M, Kratasyuk V . Immobilization of Firefly Bioluminescent System: Development and Application of Reagents. Biosensors (Basel). 2023; 13(1). PMC: 9855680. DOI: 10.3390/bios13010047. View

Bangar S, Ashogbon A, Singh A, Chaudhary V, Whiteside W . Enzymatic modification of starch: A green approach for starch applications. Carbohydr Polym. 2022; 287:119265. DOI: 10.1016/j.carbpol.2022.119265. View

Lukin I, Erezuma I, Maeso L, Zarate J, Federico Desimone M, Al-Tel T . Progress in Gelatin as Biomaterial for Tissue Engineering. Pharmaceutics. 2022; 14(6). PMC: 9229474. DOI: 10.3390/pharmaceutics14061177. View

Dubey N, Tripathi B . Nature Inspired Multienzyme Immobilization: Strategies and Concepts. ACS Appl Bio Mater. 2022; 4(2):1077-1114. DOI: 10.1021/acsabm.0c01293. View

Khan U, Selamoglu Z . Use of Enzymes in Dairy Industry: A Review of Current Progress. Arch Razi Inst. 2020; 75(1):131-136. PMC: 8410156. DOI: 10.22092/ari.2019.126286.1341. View

Choi H, Yoon J . Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals. Biosensors (Basel). 2023; 13(4). PMC: 10136675. DOI: 10.3390/bios13040492. View

Zhai X, Wu Y, Tan H . Gelatin-based Targeted Delivery Systems for Tissue Engineering. Curr Drug Targets. 2023; 24(8):673-687. DOI: 10.2174/1389450124666230605150303. View

Li J, Kong X, Ai Y . Modification of granular waxy, normal and high-amylose maize starches by maltogenic α-amylase to improve functionality. Carbohydr Polym. 2022; 290:119503. DOI: 10.1016/j.carbpol.2022.119503. View

10.

Rehm F, Chen S, Rehm B . Enzyme Engineering for In Situ Immobilization. Molecules. 2016; 21(10). PMC: 6273058. DOI: 10.3390/molecules21101370. View

11.

Hathout R, Omran M . Gelatin-based particulate systems in ocular drug delivery. Pharm Dev Technol. 2015; 21(3):379-86. DOI: 10.3109/10837450.2014.999786. View

12.

Esimbekova E, Kondik A, Kratasyuk V . Bioluminescent enzymatic rapid assay of water integral toxicity. Environ Monit Assess. 2012; 185(7):5909-16. DOI: 10.1007/s10661-012-2994-1. View

13.

Tembe S, Karve M, Inamdar S, Haram S, Melo J, DSouza S . Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film. Anal Biochem. 2005; 349(1):72-7. DOI: 10.1016/j.ab.2005.11.016. View

14.

Ashogbon A . Dual modification of various starches: Synthesis, properties and applications. Food Chem. 2020; 342:128325. DOI: 10.1016/j.foodchem.2020.128325. View

15.

Esimbekova E, Govorun A, Lonshakova-Mukina V, Kratasyuk V . Gelatin and Starch: What Better Stabilizes the Enzyme Activity?. Dokl Biol Sci. 2020; 491(1):43-46. DOI: 10.1134/S0012496620020039. View

16.

Singh R, Tiwari M, Singh R, Lee J . From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes. Int J Mol Sci. 2013; 14(1):1232-77. PMC: 3565319. DOI: 10.3390/ijms14011232. View

17.

Karacaoglu S, Timur S, Telefoncu A . Arginine selective biosensor based on arginase-urease immobilized in gelatin. Artif Cells Blood Substit Immobil Biotechnol. 2003; 31(3):357-63. DOI: 10.1081/bio-120023164. View

18.

Su K, Wang C . Recent advances in the use of gelatin in biomedical research. Biotechnol Lett. 2015; 37(11):2139-45. DOI: 10.1007/s10529-015-1907-0. View

19.

Srivastava P, Kayastha A, Srinivasan . Characterization of gelatin-immobilized pigeonpea urease and preparation of a new urea biosensor. Biotechnol Appl Biochem. 2001; 34(1):55-62. DOI: 10.1042/ba20010016. View

20.

Dong T, Zhou X, Dai Y, Yang X, Zhang W, Yu D . Application of magnetic immobilized enzyme of nano dialdehyde starch in deacidification of rice bran oil. Enzyme Microb Technol. 2022; 161:110116. DOI: 10.1016/j.enzmictec.2022.110116. View