Antarctic Soil Yeasts with Fermentative Capacity and Potential for the Wine Industry

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Dec 23

PMID 38137300

Authors

Laura Navarro

Mariona Gil I Cortiella

Ana Gutierrez-Moraga

Nancy Calisto

Cristina Ubeda

Gino Corsini

Affiliations

Soon will be listed here.

Abstract

Low fermentation temperatures are usually employed to obtain high-quality wines. This is especially interesting for white wine production since it prevents the loss of volatile compounds and a browning appearance; however, available fermentative yeasts do not usually tolerate low temperatures. Therefore, an interesting place to find new yeasts with cryotolerance is the Antarctic continent. From soil samples collected in Antarctica, 125 yeasts were isolated, of which 25 exhibited fermentative activity at 10 °C. After a fingerprinting assay, we classified the candidates into nine isotypes and sequenced internal transcribed spacer regions for their identification. These yeasts were identified as part of the genus. Sugar and alcohol tolerance tests showed that some of these Antarctic soil yeasts were able to grow up to 9% alcohol, and 25% sugar was reached; however, they exhibited longer latency periods compared to the control . The optimal growing temperature for the isolated Antarctic yeasts was between 10 °C and 15 °C. A comprehensive analysis of the results obtained showed that the isolates 10M3-1, 4M3-6, and 4B1-35 could be good candidates for fermentation purposes due to their alcohol, sugar tolerance, and growth features. Our results prove that it is possible to isolate fermentative yeasts from Antarctic soil with promising characteristics for their potential use in the wine production industry.

References

Carrasco M, Rozas J, Barahona S, Alcaino J, Cifuentes V, Baeza M . Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC Microbiol. 2012; 12:251. PMC: 3499239. DOI: 10.1186/1471-2180-12-251. View

Manzanares P, Ramon D, Querol A . Screening of non-Saccharomyces wine yeasts for the production of beta-D-xylosidase activity. Int J Food Microbiol. 2000; 46(2):105-12. DOI: 10.1016/s0168-1605(98)00186-x. View

Molina A, Swiegers J, Varela C, Pretorius I, Agosin E . Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds. Appl Microbiol Biotechnol. 2007; 77(3):675-87. DOI: 10.1007/s00253-007-1194-3. View

Linnakoski R, Jyske T, Eerikainen R, Veteli P, Cortina-Escribano M, Magalhaes F . Brewing potential of strains of the boreal wild yeast . Front Microbiol. 2023; 14:1108961. PMC: 9947644. DOI: 10.3389/fmicb.2023.1108961. View

Henrici A . The Staining of Yeasts by Gram's Method. J Med Res. 2009; 30(3):409-416.1. PMC: 2094430. View

Torija M, Beltran G, Novo M, Poblet M, Guillamon J, Mas A . Effects of fermentation temperature and Saccharomyces species on the cell fatty acid composition and presence of volatile compounds in wine. Int J Food Microbiol. 2003; 85(1-2):127-36. DOI: 10.1016/s0168-1605(02)00506-8. View

De Francesco G, Sannino C, Sileoni V, Marconi O, Filippucci S, Tasselli G . Mrakia gelida in brewing process: An innovative production of low alcohol beer using a psychrophilic yeast strain. Food Microbiol. 2018; 76:354-362. DOI: 10.1016/j.fm.2018.06.018. View

Corcoles-Saez I, Ballester-Tomas L, de la Torre-Ruiz M, Prieto J, Randez-Gil F . Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J. 2012; 446(3):477-88. DOI: 10.1042/BJ20120634. View

Penacho V, Valero E, Gonzalez R . Transcription profiling of sparkling wine second fermentation. Int J Food Microbiol. 2011; 153(1-2):176-82. DOI: 10.1016/j.ijfoodmicro.2011.11.005. View

10.

Escribano-Viana R, Gonzalez-Arenzana L, Portu J, Garijo P, Lopez-Alfaro I, Lopez R . Wine aroma evolution throughout alcoholic fermentation sequentially inoculated with non- Saccharomyces/Saccharomyces yeasts. Food Res Int. 2018; 112:17-24. DOI: 10.1016/j.foodres.2018.06.018. View

11.

Salvado Z, Arroyo-Lopez F, Guillamon J, Salazar G, Querol A, Barrio E . Temperature adaptation markedly determines evolution within the genus Saccharomyces. Appl Environ Microbiol. 2011; 77(7):2292-302. PMC: 3067424. DOI: 10.1128/AEM.01861-10. View

12.

Vaz A, Rosa L, Vieira M, de Garcia V, Brandao L, Teixeira L . The diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica. Braz J Microbiol. 2013; 42(3):937-47. PMC: 3768797. DOI: 10.1590/S1517-838220110003000012. View

13.

Strauss M, Jolly N, Lambrechts M, van Rensburg P . Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol. 2001; 91(1):182-90. DOI: 10.1046/j.1365-2672.2001.01379.x. View

14.

Beltran G, Novo M, Guillamon J, Mas A, Rozes N . Effect of fermentation temperature and culture media on the yeast lipid composition and wine volatile compounds. Int J Food Microbiol. 2007; 121(2):169-77. DOI: 10.1016/j.ijfoodmicro.2007.11.030. View

15.

Buzzini P, Branda E, Goretti M, Turchetti B . Psychrophilic yeasts from worldwide glacial habitats: diversity, adaptation strategies and biotechnological potential. FEMS Microbiol Ecol. 2012; 82(2):217-41. DOI: 10.1111/j.1574-6941.2012.01348.x. View

16.

Turchetti B, Thomas Hall S, Connell L, Branda E, Buzzini P, Theelen B . Psychrophilic yeasts from Antarctica and European glaciers: description of Glaciozyma gen. nov., Glaciozyma martinii sp. nov. and Glaciozyma watsonii sp. nov. Extremophiles. 2011; 15(5):573-86. DOI: 10.1007/s00792-011-0388-x. View

17.

Ballester-Tomas L, Prieto J, Gil J, Baeza M, Randez-Gil F . The Antarctic yeast Candida sake: Understanding cold metabolism impact on wine. Int J Food Microbiol. 2017; 245:59-65. DOI: 10.1016/j.ijfoodmicro.2017.01.009. View

18.

Diaz M, Fell J . Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genus Mrakia. Antonie Van Leeuwenhoek. 2000; 77(1):7-12. DOI: 10.1023/a:1002048008295. View

19.

Raja H, Miller A, Pearce C, Oberlies N . Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. J Nat Prod. 2017; 80(3):756-770. PMC: 5368684. DOI: 10.1021/acs.jnatprod.6b01085. View

20.

de Garcia V, Brizzio S, Libkind D, Buzzini P, van Broock M . Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. FEMS Microbiol Ecol. 2007; 59(2):331-41. DOI: 10.1111/j.1574-6941.2006.00239.x. View