Comparative Analysis of Six Chloroplast Genomes in and Its Related Genera (): New Insights into Phylogenetic Relationships and the Development of Species-Specific Molecular Markers

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Dec 23

PMID 38137004

Authors

Zixiang Wei

Fangjun Chen

Hongxia Ding

Wenli Liu

Bo Yang

Jiahui Geng

Shihua Chen

Shanli Guo

Affiliations

Soon will be listed here.

Abstract

Species within the genus hold significant research interest due to their nutritional richness and salt tolerance. However, the morphological similarities among closely related species and a dearth of genomic resources have impeded their comprehensive study and utilization. In the present research, we conduct the sequencing and assembly of chloroplast (cp) genomes from six and related species, five of which were sequenced for the first time. These genomes ranged in length from 151,850 to 152,215 base pairs, showcased typical quadripartite structures, and encoded 85 protein-coding genes (PCGs), 1 pseudogene, 37 tRNA genes, and 8 rRNA genes. Compared with the previously published sequences of related species, these cp genomes are relatively conservative, but there are also some interspecific differences, such as inversion and IR region contraction. We discerned 929 simple sequence repeats (SSRs) and a series of highly variable regions across 16 related species, predominantly situated in the intergenic spacer (IGS) region and introns. The phylogenetic evaluations revealed that is more closely related to genera such as , , , and than to other members of the family. These lineages shared a common ancestor approximately 60.80 million years ago, after which they diverged into distinct genera. Based on InDels and SNPs between species, we designed 12 pairs of primers for species identification, and experiments confirmed that they could completely distinguish 10 related species.

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