Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2023 Dec 14

PMID 38095787

Authors

Precious Earldom Mulaudzi

Heidi Abrahamse

Anine Crous

Affiliations

Soon will be listed here.

Abstract

Regenerative medicine has developed as a promising discipline that utilizes stem cells to address limitations in traditional therapies, using innovative techniques to restore and repair damaged organs and tissues. One such technique is the generation of three-dimensional (3D) organoids in stem cell therapy. Organoids are 3D constructs that resemble specific organs' structural and functional characteristics and are generated from stem cells or tissue-specific progenitor cells. The use of 3D organoids is advantageous in comparison to traditional two-dimensional (2D) cell culture by bridging the gap between in vivo and in vitro research. This review aims to provide an overview of the advancements made towards regenerative medicine using stem cells to generate organoids, explore the techniques used in generating 3D organoids and their applications and finally elucidate the challenges and future directions in regenerative medicine using 3D organoids.

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