Helium Cold Atmospheric Plasma Causes Morphological and Biochemical Alterations in Cells

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Dec 9

PMID 38067648

Authors

Sabrina de Moura Rovetta-Nogueira

Aline Chiodi Borges

Mauricio de Oliveira Filho

Thalita Mayumi Castaldelli Nishime

Luis Rogerio de Oliveira Hein

Konstantin Georgiev Kostov

Cristiane Yumi Koga-Ito

Affiliations

Soon will be listed here.

Abstract

(1) Background: Previous studies reported the promising inhibitory effect of cold atmospheric plasma (CAP) on . However, the exact mechanisms of CAP's action on the fungal cell are still poorly understood. This study aims to elucidate the CAP effect on cell wall, by evaluating the alterations on its structure and biochemical composition; (2) Methods: cells treated with Helium-CAP were analyzed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in order to detect morphological, topographic and biochemical changes in the fungal cell wall. Cells treated with caspofungin were also analyzed for comparative purposes; (3) Results: Expressive morphological and topographic changes, such as increased roughness and shape modification, were observed in the cells after CAP exposure. The alterations detected were similar to those observed after the treatment with caspofungin. The main biochemical changes occurred in polysaccharides content, and an overall decrease in glucans and an increase in chitin synthesis were detected; (4) Conclusions: Helium-CAP caused morphological and topographic alterations in cells and affected the cell wall polysaccharide content.

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