Nano/microformulations for Bacteriophage Delivery

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Dec 8

PMID 38066365

Authors

Pilar Cortes

Mary Cano-Sarabia

Joan Colom

Jennifer Otero

Daniel Maspoch

Montserrat Llagostera

Affiliations

Soon will be listed here.

Abstract

Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials: a lipid cationic mixture and a combination of alginate with the antacid CaCO. To perform bacteriophage encapsulation is necessary to dispose of a purified and highly concentrated lysate (around 10 to 10 pfu/mL) and a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy.

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