Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned CDNA

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Dec 8

PMID 38064028

Authors

Daniela Toro-Ascuy

Matias Cardenas

Yesseny Vasquez-Martinez

Marcelo Cortez-San Martin

Affiliations

Soon will be listed here.

Abstract

The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral, and mammalian genetic elements included in the pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells.

Citing Articles

Effect of eicosapentaenoic acid on innate immune responses in Atlantic salmon cells infected with infectious salmon anemia virus.

Holmlund I, Ahmadi S, Ruyter B, Ostbye T, Bou M, Gjoen T Virol J. 2025; 22(1):5.

PMID: 39780168 PMC: 11715085. DOI: 10.1186/s12985-024-02619-0.

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