A Rhizobium Meliloti Mutant That Forms Ineffective Pseudonodules in Alfalfa Produces Exopolysaccharide but Fails to Form Beta-(1----2) Glucan

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Feb 1

PMID 3804979

Citations 46

Authors

R A Geremia

S Cavaignac

A Zorreguieta

N Toro

J Olivares

R A Ugalde

Affiliations

Soon will be listed here.

Abstract

A mutant of Rhizobium meliloti that elicited the formation of inactive nodules in alfalfa was found not to form beta-(1----2) glucan in vivo or in vitro. It was nonmotile because it lacks flagella. The 235-kilodalton protein which acts as an intermediate in beta-(1----2) glucan synthesis was undetectable in the mutant. These properties of the mutant are common to those of chvB mutants of Agrobacterium tumefaciens. Exopolysaccharide formation by the R. meliloti mutant was about double that by the wild type.

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