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Biosynthesis of a Galactose-and Galacturonic Acid-containing Polysaccharide in Rhizobium Meliloti

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1986 Oct 1
PMID 3759905
Citations 7
Authors
R A Ugalde
J A Coira
W J Brill
Affiliations
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Abstract

Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R. A. Ugalde, H. Handelsman, and W. J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated galactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.

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References
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