DNA Aneuploidy in Ulcerative Colitis

Overview

Journal Gut

Specialty Gastroenterology

Date 1986 Dec 1

PMID 3804020

Citations 19

Authors

J B Fozard

P Quirke

M F Dixon

G R Giles

C C Bird

Affiliations

Soon will be listed here.

Abstract

The prevalence of deoxyribonucleic acid (DNA) aneuploidy in 297 samples from 38 patients with ulcerative colitis of varying duration was investigated by flow cytometry. In 12 patients colitis was complicated by the development of colorectal carcinoma: one had three synchronous carcinomas. Only four of 14 carcinomas were DNA aneuploid. Deoxyribonucleic acid aneuploidy occurred focally in the colorectal mucosa in the presence and absence of carcinoma: rates of aneuploidy (67% in cancer patients and 42% in non-cancer patients), were not significantly different (chi 2 = 1.0962, p = 0.295). A higher rate of DNA aneuploidy was found in dysplastic tissues (21%) compared with non-dysplastic tissues (15%), but again these differences did not reach statistical significance (chi 2 = 1.0747, p = 0.299). Deoxyribonucleic acid aneuploidy and dysplastic change occurred more often with increasing duration of ulcerative colitis (p less than 0.001, p less than 0.005 respectively). We conclude that flow cytometric analysis of cellular DNA content should not replace present morphological methods of assessment of premalignancy in ulcerative colitis, but may be a useful adjunct in the identification of abnormal mucosa.

Citing Articles

Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions.

Khalil H, Hammam O, Anis S, Elashry M, El-Yasergy D Asian Pac J Cancer Prev. 2023; 24(4):1389-1400.

PMID: 37116163 PMC: 10352723. DOI: 10.31557/APJCP.2023.24.4.1389.

Combining aneuploidy and dysplasia for colitis' cancer risk assessment outperforms current surveillance efficiency: a meta-analysis.

Meyer R, Freitag-Wolf S, Blindow S, Buning J, Habermann J Int J Colorectal Dis. 2016; 32(2):171-182.

PMID: 27766414 DOI: 10.1007/s00384-016-2684-5.

TP53/p53 alterations and Aurora A expression in progressor and non-progressor colectomies from patients with longstanding ulcerative colitis.

Friis-Ottessen M, Burum-Auensen E, Schjolberg A, Ekstrom P, Andersen S, Clausen O Int J Mol Med. 2014; 35(1):24-30.

PMID: 25333414 PMC: 4249752. DOI: 10.3892/ijmm.2014.1974.

Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis.

Friis-Ottessen M, DE Angelis P, Schjolberg A, Andersen S, Clausen O Int J Mol Med. 2014; 33(6):1477-83.

PMID: 24676865 PMC: 4055619. DOI: 10.3892/ijmm.2014.1708.

Telomere shortening correlates to dysplasia but not to DNA aneuploidy in longstanding ulcerative colitis.

Friis-Ottessen M, Bendix L, Kolvraa S, Norheim-Andersen S, DE Angelis P, Clausen O BMC Gastroenterol. 2014; 14:8.

PMID: 24405569 PMC: 3893461. DOI: 10.1186/1471-230X-14-8.

References

de Dombal F, Watts J, Watkinson G, Goligher J . Local complications of ulcerative colitis: stricture, pseudopolyposis, and carcinoma of colon and rectum. Br Med J. 1966; 1(5501):1442-7. PMC: 1844640. DOI: 10.1136/bmj.1.5501.1442. View

Quirke P, Fozard J, Dixon M, Dyson J, Giles G, Bird C . DNA aneuploidy in colorectal adenomas. Br J Cancer. 1986; 53(4):477-81. PMC: 2001435. DOI: 10.1038/bjc.1986.75. View

KEWENTER J, Ahlman H, Hulten L . Cancer risk in extensive ulcerative colitis. Ann Surg. 1978; 188(6):824-8. PMC: 1396996. DOI: 10.1097/00000658-197812000-00018. View

Blackstone M, Riddell R, Rogers B, Levin B . Dysplasia-associated lesion or mass (DALM) detected by colonoscopy in long-standing ulcerative colitis: an indication for colectomy. Gastroenterology. 1981; 80(2):366-74. View

Tribukait B, Hammarberg C, Rubio C . Ploidy and proliferation patterns in colo-rectal adenocarcinomas related to Dukes' classification and to histopathological differentiation. A flow-cytometric DNA study. Acta Pathol Microbiol Immunol Scand A. 1983; 91(2):89-95. View

Lennard-Jones J, MORSON B, Ritchie J, Williams C . Cancer surveillance in ulcerative colitis. Experience over 15 years. Lancet. 1983; 2(8342):149-52. DOI: 10.1016/s0140-6736(83)90129-0. View

Hedley D, Friedlander M, TAYLOR I, Rugg C, Musgrove E . Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983; 31(11):1333-5. DOI: 10.1177/31.11.6619538. View

Riddell R, Goldman H, Ransohoff D, Appelman H, Fenoglio C, Haggitt R . Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol. 1983; 14(11):931-68. DOI: 10.1016/s0046-8177(83)80175-0. View

Hammarberg C, Slezak P, Tribukait B . Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984; 53(2):291-5. DOI: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. View

10.

Boland C, Lance P, Levin B, Riddell R, Kim Y . Abnormal goblet cell glycoconjugates in rectal biopsies associated with an increased risk of neoplasia in patients with ulcerative colitis: early results of a prospective study. Gut. 1984; 25(12):1364-71. PMC: 1420186. DOI: 10.1136/gut.25.12.1364. View

11.

Hendriksen C, Kreiner S, Binder V . Long term prognosis in ulcerative colitis--based on results from a regional patient group from the county of Copenhagen. Gut. 1985; 26(2):158-63. PMC: 1432417. DOI: 10.1136/gut.26.2.158. View

12.

Allen D, BIGGART J, Orchin J, Foster H . An immunoperoxidase study of epithelial marker antigens in ulcerative colitis with dysplasia and carcinoma. J Clin Pathol. 1985; 38(1):18-29. PMC: 499067. DOI: 10.1136/jcp.38.1.18. View

13.

Ehsanullah M, Naunton Morgan M, FILIPE M, Gazzard B . Sialomucins in the assessment of dysplasia and cancer-risk patients with ulcerative colitis treated with colectomy and ileo-rectal anastomosis. Histopathology. 1985; 9(2):223-35. DOI: 10.1111/j.1365-2559.1985.tb02437.x. View

14.

van den Ingh H, Griffioen G, Cornelisse C . Flow cytometric detection of aneuploidy in colorectal adenomas. Cancer Res. 1985; 45(7):3392-7. View

15.

Shields H, Bates M, Goldman H, Zuckerman G, Mills B, Best C . Scanning electron microscopic appearance of chronic ulcerative colitis with and without dysplasia. Gastroenterology. 1985; 89(1):62-72. DOI: 10.1016/0016-5085(85)90746-2. View

16.

Hedley D, Friedlander M, TAYLOR I . Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry. 1985; 6(4):327-33. DOI: 10.1002/cyto.990060409. View

17.

Armitage N, Robins R, Evans D, Turner D, Baldwin R, Hardcastle J . The influence of tumour cell DNA abnormalities on survival in colorectal cancer. Br J Surg. 1985; 72(10):828-30. DOI: 10.1002/bjs.1800721018. View

18.

Lennard-Jones J, MORSON B, Ritchie J, Shove D, Williams C . Cancer in colitis: assessment of the individual risk by clinical and histological criteria. Gastroenterology. 1977; 73(6):1280-9. View