Flow Cytometric DNA Ploidy Pattern in Dysplastic Mucosa, and in Primary and Metastatic Carcinomas in Patients with Longstanding Ulcerative Colitis

Overview

Journal Br J Cancer

Specialty Oncology

Date 1991 Aug 1

PMID 1892760

Citations 8

Authors

G I Meling

O P Clausen

A Bergan

A Schjolberg

T O Rognum

Affiliations

Soon will be listed here.

Abstract

Eighty-nine fresh tissue samples from flat colonic mucosa, polypoid lesions, macroscopically evident carcinomas, and metastatic carcinomas from ten patients with longstanding ulcerative colitis (greater than or equal to 8 years duration) were analysed by DNA flow cytometry and light microscopy. Of a total of ten carcinomas found in six patients, six showed DNA aneuploidy. Three patients developed metastatic carcinomas, all with aneuploid cell populations with similar DNA indices as in the primary carcinoma. Furthermore, aneuploid cell populations with similar DNA indices often occurred, both in separate mucosa samples, as well as in mucosa and carcinoma samples, from the same patient. DNA aneuploidy was found in flat mucosa in five of the six patients with carcinoma, and in one of the four patients without carcinoma (P greater than 0.1). High grade dysplasia was found in only four of the six cases with carcinoma, indicating that high grade dysplasia is insufficient as marker for malignant development. DNA aneuploidy was found in 24% of the dysplastic mucosa samples, and in 18% of the non-dysplastic mucosa samples (n.s., both with regard to high and low grade dysplasia). Since abnormal DNA ploidy pattern was not confined to dysplastic epithelium only, DNA aneuploidy in flat mucosa may constitute an additional marker in the identification of patients at increased cancer risk who could benefit from a closer surveillance.

Citing Articles

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TP53/p53 alterations and Aurora A expression in progressor and non-progressor colectomies from patients with longstanding ulcerative colitis.

Friis-Ottessen M, Burum-Auensen E, Schjolberg A, Ekstrom P, Andersen S, Clausen O Int J Mol Med. 2014; 35(1):24-30.

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Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis.

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Telomere shortening correlates to dysplasia but not to DNA aneuploidy in longstanding ulcerative colitis.

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