Bacteria-Based Living Probes: Preparation and the Applications in Bioimaging and Diagnosis

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2023 Nov 30

PMID 38032119

Authors

Hejin Jiang

Zhenping Cao

Ying Liu

Rui Liu

Yan Zhou

Jinyao Liu

Affiliations

Soon will be listed here.

Abstract

Bacteria can colonize a variety of in vivo biointerfaces, particularly the skin, nasal, and oral mucosa, the gastrointestinal tract, and the reproductive tract, but also target specific lesion sites, such as tumor and wound. By virtue of their prominent characteristics in motility, editability, and targeting ability, bacteria carrying imageable agents are widely developed as living probes for bioimaging and diagnosis of different diseases. This review first introduces the strategies used for preparing bacteria-based living probes, including biological engineering, chemical modification, intracellular loading, and optical manipulation. It then summarizes the recent progress of these living probes for fluorescence imaging, near-infrared imaging, ultrasonic imaging, photoacoustic imaging, magnetic resonance imaging, and positron emission tomography imaging. The biomedical applications of bacteria-based living probes are also reviewed particularly in the bioimaging and diagnosis of bacterial infections, cancers, and intestine-associated diseases. In addition, the advantages and challenges of bacteria-based living probes are discussed and future perspectives are also proposed. This review provides an updated overview of bacteria-based living probes, highlighting their great potential as a unique yet versatile platform for developing next-generation imageable agents for intelligent bioimaging, diagnosis, and even therapy.

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References

Kotakadi S, Borelli D, Nannepaga J . Therapeutic Applications of Magnetotactic Bacteria and Magnetosomes: A Review Emphasizing on the Cancer Treatment. Front Bioeng Biotechnol. 2022; 10:789016. PMC: 9081342. DOI: 10.3389/fbioe.2022.789016. View

Li Y, Hu X, Ding D, Zou Y, Xu Y, Wang X . In situ targeted MRI detection of Helicobacter pylori with stable magnetic graphitic nanocapsules. Nat Commun. 2017; 8:15653. PMC: 5501158. DOI: 10.1038/ncomms15653. View

Wu H, Moser C, Wang H, Hoiby N, Song Z . Strategies for combating bacterial biofilm infections. Int J Oral Sci. 2014; 7(1):1-7. PMC: 4817533. DOI: 10.1038/ijos.2014.65. View

Li N, Cannon M . Gas vesicle genes identified in Bacillus megaterium and functional expression in Escherichia coli. J Bacteriol. 1998; 180(9):2450-8. PMC: 107188. DOI: 10.1128/JB.180.9.2450-2458.1998. View

Chen Y, Xu M, Xu W, Song H, Hu L, Xue S . Highly selective and wash-free visualization of resistant bacteria with a relebactam-derived fluorogenic probe. Chem Commun (Camb). 2019; 55(67):9919-9922. DOI: 10.1039/c9cc04533c. View

Persat A, Nadell C, Kim M, Ingremeau F, Siryaporn A, Drescher K . The mechanical world of bacteria. Cell. 2015; 161(5):988-997. PMC: 4451180. DOI: 10.1016/j.cell.2015.05.005. View

Yuan H, Liu Z, Liu L, Lv F, Wang Y, Wang S . Cationic conjugated polymers for discrimination of microbial pathogens. Adv Mater. 2014; 26(25):4333-8. DOI: 10.1002/adma.201400636. View

Dholakia K, Reece P, Gu M . Optical micromanipulation. Chem Soc Rev. 2008; 37(1):42-55. DOI: 10.1039/b512471a. View

Xin H, Li Y, Liu X, Li B . Escherichia coli-based biophotonic waveguides. Nano Lett. 2013; 13(7):3408-13. DOI: 10.1021/nl401870d. View

10.

Wu M, Wu W, Duan Y, Liu X, Wang M, Phan C . HClO-Activated Fluorescence and Photosensitization from an AIE Nanoprobe for Image-Guided Bacterial Ablation in Phagocytes. Adv Mater. 2020; 32(47):e2005222. DOI: 10.1002/adma.202005222. View

11.

Avci P, Karimi M, Sadasivam M, Antunes-Melo W, Carrasco E, Hamblin M . In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging. Virulence. 2017; 9(1):28-63. PMC: 6067836. DOI: 10.1080/21505594.2017.1371897. View

12.

Kong Y, Jiang Q, Zhang F, Yang Y . Small Molecular Fluorescent Probes: Application Progress of Specific Bacteria Detection and Antibacterial Phototherapy. Chem Asian J. 2023; 18(11):e202300178. DOI: 10.1002/asia.202300178. View

13.

Xu W, Su P, Zheng L, Fan H, Wang Y, Liu Y . Imaging of a Novel Strain of via Metabolic Labeling. Front Microbiol. 2018; 9:2298. PMC: 6174215. DOI: 10.3389/fmicb.2018.02298. View

14.

Mota F, Jain S . Flagging Bacteria with Radiolabeled d-Amino Acids. ACS Cent Sci. 2020; 6(2):97-99. PMC: 7047284. DOI: 10.1021/acscentsci.0c00096. View

15.

Zhang Q, Song B, Xu Y, Yang Y, Ji J, Cao W . In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter. Nat Commun. 2023; 14(1):2331. PMC: 10122673. DOI: 10.1038/s41467-023-37827-9. View

16.

Xin H, Xu R, Li B . Optical trapping, driving, and arrangement of particles using a tapered fibre probe. Sci Rep. 2012; 2:818. PMC: 3495291. DOI: 10.1038/srep00818. View

17.

Baker R, Mahmud A, Miller I, Rajeev M, Rasambainarivo F, Rice B . Infectious disease in an era of global change. Nat Rev Microbiol. 2021; 20(4):193-205. PMC: 8513385. DOI: 10.1038/s41579-021-00639-z. View

18.

Kwon S, Jiang S, Zheng J, Choy H, Min J . Rhodobacter sphaeroides, a novel tumor-targeting bacteria that emits natural near-infrared fluorescence. Microbiol Immunol. 2014; 58(3):172-9. DOI: 10.1111/1348-0421.12134. View

19.

Paulini F, Marangon A, Azevedo C, Brito J, Lemos M, Sousa M . In Vivo Evaluation of DMSA-Coated Magnetic Nanoparticle Toxicity and Biodistribution in Rats: A Long-Term Follow-Up. Nanomaterials (Basel). 2022; 12(19). PMC: 9565739. DOI: 10.3390/nano12193513. View

20.

Gautam S, Gniadek T, Kim T, Spiegel D . Exterior design: strategies for redecorating the bacterial surface with small molecules. Trends Biotechnol. 2013; 31(4):258-67. PMC: 3641905. DOI: 10.1016/j.tibtech.2013.01.012. View