Cardioprotection of Water Spinach (), Wood Apple () and Linseed ( L.) on Doxorubicin-Induced Cardiotoxicity and Oxidative Stress in Rat Model

Overview

Journal Nutr Metab Insights

Publisher Sage Publications

Specialty Endocrinology

Date 2023 Nov 29

PMID 38024869

Authors

Maisha Majid Mukta

Md Jamal Hossain

Mousumi Akter

Badhan Banik

Md Mahbub Zaman Mithun

Sneha Sarwar

Md Saidul Arefin

Md Rabiul Islam

Sheikh Nazrul Islam

Affiliations

Soon will be listed here.

Abstract

Objectives: The aim of this study was to investigate the pharmacological efficacy of 3 functional foods (Water spinach, Wood apple, and Linseed) against doxorubicin-induced cardiotoxicity and oxidative stress in rat models.

Methods: Twenty-five Wistar Albino rats (male and female) were equally classified into 5 groups. Except for the normal control (NC) group, the animals received 2.5 mg/kg doxorubicin (DOX) intra-peritoneal injection at 48 hours intervals to create a dose of 15 mg/kg overall for 14 days. Simply a standard diet was given to the NC and DOX groups. In the 3 treatment groups such as water spinach (DOX + WS), wood apple (DOX + WA), and linseed (DOX + LS), rats were given 14 gm/day/rat fried water spinach, mashed wood apple, roasted linseed, respectively mixed with regular rat diet at 1:1 ratio. Blood and heart samples were collected by sacrificing all the rats on the last of the experiment day (the 15 day). LDH (lactate dehydrogenase), CK-MB (creatine kinase myocardial band), MDA (malondialdehyde), and SOD (superoxide dismutase) were analyzed. Additionally, histopathological analysis was conducted for final observation.

Results: The functional foods were indicated to lower the serum cardiac biomarkers (LDH and CK-MB) as well as stress marker (MDA) significantly ( < .05) and improved heart function and oxidative stress. However, the change in serum SOD level was noted as statistically insignificant ( > .05). The biochemical outcomes of the food intervention groups were supported by the histological findings found in those groups.

Conclusion: Consuming the investigated foods containing antioxidant phytochemicals may combat cardiac toxicity and oxidative stress. Nonetheless, thorough investigations and clinical monitoring are required to understand these functional foods' mechanism of action and dose-response effects in treating cardiotoxicity and oxidative stress.

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