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Sample Size Calculation in Animal Studies Using Resource Equation Approach

Overview
Journal Malays J Med Sci
Specialty General Medicine
Date 2018 Feb 2
PMID 29386977
Citations 238
Authors
Wan Nor Arifin
Wan Mohd Zahiruddin
Affiliations
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Abstract

Animal research plays an important role in the pre-clinical phase of clinical trials. In animal studies, the power analysis approach to sample size calculation is recommended. Whenever it is not possible to assume the standard deviation and the effect size, an alternative to the power analysis approach is the 'resource equation' approach, which sets the acceptable range of the error degrees of freedom (DF) in an analysis of variance (ANOVA). The aim of this article is to guide researchers in calculating the minimum and maximum numbers of animals required in animal research by reformulating the error DF formulas.

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References
1.
Charan J, Kantharia N . How to calculate sample size in animal studies?. J Pharmacol Pharmacother. 2013; 4(4):303-6. PMC: 3826013. DOI: 10.4103/0976-500X.119726. View
2.
Festing M, Altman D . Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J. 2002; 43(4):244-58. DOI: 10.1093/ilar.43.4.244. View
3.
Muhlhausler B, Bloomfield F, Gillman M . Whole animal experiments should be more like human randomized controlled trials. PLoS Biol. 2013; 11(2):e1001481. PMC: 3570551. DOI: 10.1371/journal.pbio.1001481. View
4.
Fitts D . Ethics and animal numbers: informal analyses, uncertain sample sizes, inefficient replications, and type I errors. J Am Assoc Lab Anim Sci. 2011; 50(4):445-53. PMC: 3148647. View
5.
Festing M . Design and statistical methods in studies using animal models of development. ILAR J. 2006; 47(1):5-14. DOI: 10.1093/ilar.47.1.5. View