Anticoccidial Effects of Tannin-based Herbal Formulation (, , and ) Against Coccidiosis in Broilers

Overview

Journal J Parasit Dis

Specialty Parasitology

Date 2023 Nov 27

PMID 38009158

Authors

Seyed Ali Ghafouri

Abolfazl Ghaniei

Soheil Sadr

Amir Ali Amiri

Amir Ebrahim Tavanaee Tamannaei

Ali Charbgoo

Shakila Ghiassi

Behnoush Dianat

Affiliations

Soon will be listed here.

Abstract

Background: Avian coccidiosis is considered among the infectious disease of high cost in the poultry industry. Herbal extracts are safe and reliable substitute anticoccidial drugs for chemical feed additives as they do not sequel to drug resistance and tissue remnants.

Objective: The current study aimed to assess the anticoccidial effect of an herbal complex of 3 plants (, , and ) in broiler chickens compared to toltrazuril anticoccidial.

Methods: This experiment used one hundred twenty broiler chickens and divided them into four equally numbered groups. All the groups, except group (D), were experimentally infected with mixed spp. (. , , and ) on day 14. Group (A) was treated with a herbal mixture, containing 75% with a minimum of 30% total tannin, 16% with a minimum of 0.02% artemisinin, and 9% with a minimum of 0.4% total phenol contents. Group (B) was treated with toltrazuril. Group (C) did not have any treatment. Group (D) was healthy all the experiment period as a negative control group. During a 42-day breeding period, the study examined clinical signs, weight gains, feed conversion ratio, lesions scoring, casualties, and the number of oocysts in different bird groups.

Results: Group (D) showed the most significant weight gain, indicating the economic damage caused by coccidiosis. The best feed conversion ratio was observed in the unchallenged group, and coccidiosis negatively affected it in other groups. Clinical signs of dysentery, diarrhea, and lethargy were seen post-challenge but improved with treatment. Group (D) showed no losses; others had casualties and coccidiosis lesions. Lesion scores were lowest in the group (D), and the herbal mixture improved performance. The herbal mixture and toltrazuril reduced oocyst counts in feces earlier than the untreated group.

Conclusion: In conclusion, the anticoccidial activity of the mentioned herbal complex recommends its use as an alternative anticoccidial agent to chemotherapeutic drugs for controlling coccidiosis.

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References

Elham A, Arken M, Kalimanjan G, Arkin A, Iminjan M . A review of the phytochemical, pharmacological, pharmacokinetic, and toxicological evaluation of Quercus Infectoria galls. J Ethnopharmacol. 2020; 273:113592. DOI: 10.1016/j.jep.2020.113592. View

Remmal A, Achahbar S, Bouddine L, Chami N, Chami F . In vitro destruction of Eimeria oocysts by essential oils. Vet Parasitol. 2011; 182(2-4):121-6. DOI: 10.1016/j.vetpar.2011.06.002. View

Muthamilselvan T, Kuo T, Wu Y, Yang W . Herbal Remedies for Coccidiosis Control: A Review of Plants, Compounds, and Anticoccidial Actions. Evid Based Complement Alternat Med. 2016; 2016:2657981. PMC: 4939967. DOI: 10.1155/2016/2657981. View

Youn H, Noh J . Screening of the anticoccidial effects of herb extracts against Eimeria tenella. Vet Parasitol. 2001; 96(4):257-63. DOI: 10.1016/s0304-4017(01)00385-5. View

Arafat N, Abbas I . Coccidia of Japanese Quail: From Identification, Prevalence, Infection, and Immunization. J Parasitol. 2017; 104(1):23-30. DOI: 10.1645/17-109. View

Van Der Kooy F, Sullivan S . The complexity of medicinal plants: the traditional Artemisia annua formulation, current status and future perspectives. J Ethnopharmacol. 2013; 150(1):1-13. DOI: 10.1016/j.jep.2013.08.021. View

Johnson J, REID W . Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol. 1970; 28(1):30-6. DOI: 10.1016/0014-4894(70)90063-9. View

Kim D, Lillehoj H, Lee S, Lillehoj E, Bravo D . Improved resistance to Eimeria acervulina infection in chickens due to dietary supplementation with garlic metabolites. Br J Nutr. 2012; 109(1):76-88. DOI: 10.1017/S0007114512000530. View

Kothari D, Lee W, Niu K, Kim S . The Genus as Poultry Feed Additive: A Review. Animals (Basel). 2019; 9(12). PMC: 6940947. DOI: 10.3390/ani9121032. View

10.

Tonda R, Rubach J, Lumpkins B, Mathis G, Poss M . Effects of tannic acid extract on performance and intestinal health of broiler chickens following coccidiosis vaccination and/or a mixed-species Eimeria challenge. Poult Sci. 2018; 97(9):3031-3042. DOI: 10.3382/ps/pey158. View

11.

Feng X, Cao S, Qiu F, Zhang B . Traditional application and modern pharmacological research of Artemisia annua L. Pharmacol Ther. 2020; 216:107650. DOI: 10.1016/j.pharmthera.2020.107650. View

12.

Dorrigiv M, Zareiyan A, Hosseinzadeh H . Garlic (Allium sativum) as an antidote or a protective agent against natural or chemical toxicities: A comprehensive update review. Phytother Res. 2020; 34(8):1770-1797. DOI: 10.1002/ptr.6645. View

13.

Elmahallawy E, Fehaid A, El-Shewehy D, Ramez A, Alkhaldi A, Mady R . S-Methylcysteine Ameliorates the Intestinal Damage Induced by Infection Targeting Oxidative Stress and Inflammatory Modulators. Front Vet Sci. 2022; 8:754991. PMC: 8767015. DOI: 10.3389/fvets.2021.754991. View

14.

Qaid M, Al-Mufarrej S, Azzam M, Al-Garadi M . Anticoccidial effectivity of a traditional medicinal plant, Cinnamomum verum, in broiler chickens infected with Eimeria tenella. Poult Sci. 2021; 100(3):100902. PMC: 7936149. DOI: 10.1016/j.psj.2020.11.071. View

15.

Ghafouri S, Ghaniei A, Tavanaee Tamannaei A, Sadr S, Charbgoo A, Ghiassi S . Evaluation of therapeutic effects of an herbal mixture (Echinacea purpurea and Glycyrrhiza glabra) for treatment of clinical coccidiosis in broilers. Vet Med Sci. 2022; 9(2):829-836. PMC: 10029886. DOI: 10.1002/vms3.971. View

16.

Gotep J, Tanko J, Forcados G, Muraina I, Ozele N, Dogonyaro B . Therapeutic and Safety Evaluation of Combined Aqueous Extracts of Azadirachta indica and Khaya senegalensis in Chickens Experimentally Infected with Eimeria Oocysts. J Parasitol Res. 2016; 2016:4692424. PMC: 4771916. DOI: 10.1155/2016/4692424. View

17.

Haug A, Williams R, Larsen S . Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method. Vet Parasitol. 2006; 136(3-4):233-42. DOI: 10.1016/j.vetpar.2005.11.024. View

18.

Allen P, Lydon J, Danforth H . Effects of components of Artemisia annua on coccidia infections in chickens. Poult Sci. 1997; 76(8):1156-63. DOI: 10.1093/ps/76.8.1156. View

19.

Abu-Akkada S, Oda S, Ashmawy K . Garlic and hepatic coccidiosis: prophylaxis or treatment?. Trop Anim Health Prod. 2010; 42(7):1337-43. DOI: 10.1007/s11250-010-9590-6. View

20.

Chand N, Faheem H, Khan R, Subhan Qureshi M, A Alhidary I, Abudabos A . Anticoccidial effect of mananoligosacharide against experimentally induced coccidiosis in broiler. Environ Sci Pollut Res Int. 2016; 23(14):14414-21. DOI: 10.1007/s11356-016-6600-x. View