Atypical Protein Kinase C Promotes Its Own Asymmetric Localisation by Phosphorylating Cdc42 in the Zygote

Overview

Journal bioRxiv

Date 2023 Nov 27

PMID 38009101

Authors

John Packer

Alicia G Gubieda

Aaron Brooks

Lars N Deutz

Iolo Squires

Shona Ellison

Claudia Schneider

Sundar Ram Naganathan

Adam J M Wollman

Daniel J Dickinson

Josana Rodriguez

Affiliations

Soon will be listed here.

Abstract

Atypical protein kinase C (aPKC) is a major regulator of cell polarity. Acting in conjunction with Par6, Par3 and the small GTPase Cdc42, aPKC becomes asymmetrically localised and drives the polarisation of cells. aPKC activity is crucial for its own asymmetric localisation, suggesting a hitherto unknown feedback mechanism contributing to polarisation. Here we show in the zygote that the feedback relies on aPKC phosphorylation of Cdc42 at serine 71. The turnover of CDC-42 phosphorylation ensures optimal aPKC asymmetry and activity throughout polarisation by tuning Par6/aPKC association with Par3 and Cdc42. Moreover, turnover of Cdc42 phosphorylation regulates actomyosin cortex dynamics that are known to drive aPKC asymmetry. Given the widespread role of aPKC and Cdc42 in cell polarity, this form of self-regulation of aPKC may be vital for the robust control of polarisation in many cell types.

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