NMY-2 Maintains Cellular Asymmetry and Cell Boundaries, and Promotes a SRC-dependent Asymmetric Cell Division

Overview

Journal Dev Biol

Publisher Elsevier

Specialties Biology
Reproductive Medicine

Date 2010 Jan 12

PMID 20059995

Citations 35

Authors

Ji Liu

Lisa L Maduzia

Masaki Shirayama

Craig C Mello

Affiliations

Soon will be listed here.

Abstract

The nonmuscle myosin II NMY-2 is required for cytokinesis as well as for the establishment of zygote asymmetry during embryogenesis in Caenorhabditis elegans. Here we describe two conditional nmy-2 alleles that rapidly and reversibly inactivate the protein. We show that NMY-2 has late-cell-cycle roles in maintaining embryonic asymmetries and is also required for a surprisingly late step in the maintenance of the cytokinesis furrow. Finally, during a signaling-induced asymmetric cell division, NMY-2 is required for SRC-dependent phosphotyrosine signaling and acts in parallel with WNT-signaling to specify endoderm.

Citing Articles

myh9b is a critical non-muscle myosin II encoding gene that interacts with myh9a and myh10 during zebrafish development in both compensatory and redundant pathways.

Rolfs L, Falat E, Gutzman J G3 (Bethesda). 2024; 15(1.

PMID: 39503257 PMC: 11708221. DOI: 10.1093/g3journal/jkae260.

Cell type-specific regulation by different cytokinetic pathways in the early embryo.

Connors C, Martin S, Dumont J, Shirasu-Hiza M, Canman J MicroPubl Biol. 2024; 2024.

PMID: 39502424 PMC: 11536048. DOI: 10.17912/micropub.biology.001316.

Actomyosin-mediated apical constriction promotes physiological germ cell death in C. elegans.

Kohlbrenner T, Berger S, Laranjeira A, Aegerter-Wilmsen T, Comi L, DeMello A PLoS Biol. 2024; 22(8):e3002775.

PMID: 39178318 PMC: 11376560. DOI: 10.1371/journal.pbio.3002775.

A cytokinetic ring-driven cell rotation achieves Hertwig's rule in early development.

Middelkoop T, Neipel J, Cornell C, Naumann R, Pimpale L, Julicher F Proc Natl Acad Sci U S A. 2024; 121(25):e2318838121.

PMID: 38870057 PMC: 11194556. DOI: 10.1073/pnas.2318838121.

NMY-2, TOE-2 and PIG-1 regulate Caenorhabditis elegans asymmetric cell divisions.

Robinson J, Teuliere J, Yoo S, Garriga G PLoS One. 2024; 19(5):e0304064.

PMID: 38787850 PMC: 11125515. DOI: 10.1371/journal.pone.0304064.

References

Bjorge J, Jakymiw A, Fujita D . Selected glimpses into the activation and function of Src kinase. Oncogene. 2000; 19(49):5620-35. DOI: 10.1038/sj.onc.1203923. View

Schlesinger A, Shelton C, Maloof J, Meneghini M, Bowerman B . Wnt pathway components orient a mitotic spindle in the early Caenorhabditis elegans embryo without requiring gene transcription in the responding cell. Genes Dev. 1999; 13(15):2028-38. PMC: 316921. DOI: 10.1101/gad.13.15.2028. View

Hill D, Strome S . An analysis of the role of microfilaments in the establishment and maintenance of asymmetry in Caenorhabditis elegans zygotes. Dev Biol. 1988; 125(1):75-84. DOI: 10.1016/0012-1606(88)90060-7. View

Liu J, Vasudevan S, Kipreos E . CUL-2 and ZYG-11 promote meiotic anaphase II and the proper placement of the anterior-posterior axis in C. elegans. Development. 2004; 131(15):3513-25. DOI: 10.1242/dev.01245. View

Bei Y, Hogan J, Berkowitz L, Soto M, Rocheleau C, Pang K . SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos. Dev Cell. 2002; 3(1):113-25. DOI: 10.1016/s1534-5807(02)00185-5. View

Goldstein B . Establishment of gut fate in the E lineage of C. elegans: the roles of lineage-dependent mechanisms and cell interactions. Development. 1993; 118(4):1267-77. DOI: 10.1242/dev.118.4.1267. View

Cuenca A, Schetter A, Aceto D, Kemphues K, Seydoux G . Polarization of the C. elegans zygote proceeds via distinct establishment and maintenance phases. Development. 2003; 130(7):1255-65. PMC: 1761648. DOI: 10.1242/dev.00284. View

Goldstein B, Macara I . The PAR proteins: fundamental players in animal cell polarization. Dev Cell. 2007; 13(5):609-622. PMC: 2964935. DOI: 10.1016/j.devcel.2007.10.007. View

Pang K, Ishidate T, Nakamura K, Shirayama M, Trzepacz C, Schubert C . The minibrain kinase homolog, mbk-2, is required for spindle positioning and asymmetric cell division in early C. elegans embryos. Dev Biol. 2003; 265(1):127-39. DOI: 10.1016/j.ydbio.2003.09.024. View

10.

Tama F, Feig M, Liu J, Brooks 3rd C, Taylor K . The requirement for mechanical coupling between head and S2 domains in smooth muscle myosin ATPase regulation and its implications for dimeric motor function. J Mol Biol. 2004; 345(4):837-54. DOI: 10.1016/j.jmb.2004.10.084. View

11.

Boyd L, Guo S, Levitan D, Stinchcomb D, Kemphues K . PAR-2 is asymmetrically distributed and promotes association of P granules and PAR-1 with the cortex in C. elegans embryos. Development. 1996; 122(10):3075-84. DOI: 10.1242/dev.122.10.3075. View

12.

Thorpe C, Schlesinger A, Carter J, Bowerman B . Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm. Cell. 1997; 90(4):695-705. DOI: 10.1016/s0092-8674(00)80530-9. View

13.

Goldstein B . An analysis of the response to gut induction in the C. elegans embryo. Development. 1995; 121(4):1227-36. DOI: 10.1242/dev.121.4.1227. View

14.

Goldstein B . Induction of gut in Caenorhabditis elegans embryos. Nature. 1992; 357(6375):255-7. DOI: 10.1038/357255a0. View

15.

Nakamura K, Kim S, Ishidate T, Bei Y, Pang K, Shirayama M . Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. Genes Dev. 2005; 19(15):1749-54. PMC: 1182335. DOI: 10.1101/gad.1323705. View

16.

Bowerman B, Shelton C . Cell polarity in the early Caenorhabditis elegans embryo. Curr Opin Genet Dev. 1999; 9(4):390-5. DOI: 10.1016/S0959-437X(99)80059-8. View

17.

Hung T, Kemphues K . PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos. Development. 1998; 126(1):127-35. DOI: 10.1242/dev.126.1.127. View

18.

Rocheleau C, Downs W, Lin R, Wittmann C, Bei Y, Cha Y . Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos. Cell. 1997; 90(4):707-16. DOI: 10.1016/s0092-8674(00)80531-0. View

19.

Gromley A, Yeaman C, Rosa J, Redick S, Chen C, Mirabelle S . Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. Cell. 2005; 123(1):75-87. DOI: 10.1016/j.cell.2005.07.027. View

20.

Munro E, Nance J, Priess J . Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryo. Dev Cell. 2004; 7(3):413-24. DOI: 10.1016/j.devcel.2004.08.001. View