A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Nov 25

PMID 38006562

Authors

Laura Renee Serbus

Affiliations

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Abstract

The success of microbial endosymbionts, which reside naturally within a eukaryotic "host" organism, requires effective microbial interaction with, and manipulation of, the host cells. Fluorescence microscopy has played a key role in elucidating the molecular mechanisms of endosymbiosis. For 30 years, fluorescence analyses have been a cornerstone in studies of endosymbiotic Wolbachia bacteria, focused on host colonization, maternal transmission, reproductive parasitism, horizontal gene transfer, viral suppression, and metabolic interactions in arthropods and nematodes. Fluorescence-based studies stand to continue informing Wolbachia-host interactions in increasingly detailed and innovative ways.

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