Adipose-Derived Mesenchymal Stem Cell (MSC) Immortalization by Modulation of and Expression Levels

Overview

Journal J Pers Med

Date 2023 Nov 25

PMID 38003936

Authors

Aigul R Rakhmatullina

Rimma N Mingaleeva

Dina U Gafurbaeva

Olesya N Glazunova

Aisylu R Sagdeeva

Emil R Bulatov

Albert A Rizvanov

Regina R Miftakhova

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells (MSCs) are pivotal players in tissue repair and hold great promise as cell therapeutic agents for regenerative medicine. Additionally, they play a significant role in the development of various human diseases. Studies on MSC biology have encountered a limiting property of these cells, which includes a low number of passages and a decrease in differentiation potential during in vitro culture. Although common methods of immortalization through gene manipulations of cells are well established, the resulting MSCs vary in differentiation potential compared to primary cells and eventually undergo senescence. This study aimed to immortalize primary adipose-derived MSCs by overexpressing human telomerase reverse transcriptase () gene combined with a knockdown of . The research demonstrated that immortalized MSCs maintained a stable level of differentiation into osteogenic and chondrogenic lineages during 30 passages, while also exhibiting an increase in cell proliferation rate and differentiation potential towards the adipogenic lineage. Long-term culture of immortalized cells did not alter cell morphology and self-renewal potential. Consequently, a genetically stable line of immortalized adipose-derived MSCs (iMSCs) was established.

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