Expression of Genes Involved in Anthracnose Resistance in Chili () 'PBC80'-Derived Recombinant Inbred Lines

Overview

Journal Pathogens

Date 2023 Nov 25

PMID 38003772

Authors

Wassana Kethom

Paul W J Taylor

Orarat Mongkolporn

Affiliations

Soon will be listed here.

Abstract

Chili anthracnose has long been a threat to chili production worldwide. 'PBC80' has been identified as a source of resistance to anthracnose. Recently, a QTL for ripe fruit resistance from 'PBC80'-derived RILs was located on chromosome 4 (123 Mb) and contained over 80 defense-related genes. To identify the genes most related to anthracnose resistance, a fine map of the QTL region was developed using single-marker analysis. Nine genes were selected from the new QTL (1.12 Mb) to study their expression after being challenged with 'MJ5' in two different RIL genotypes (Resistance/Resistance or R/R and Susceptible/Susceptible or S/S) at 0, 6 and 12 h. Of the nine genes, , , , , and were significantly up-regulated, compared to the control, in the R/R genotype. was up-regulated in both chili genotypes. However, the expression was relatively and constantly low in the S/S genotype. Most up-regulated genes reached the highest peak (2.3-4.5 fold) at 6 h, except for , which had the highest peak at 12 h (6.4 fold). The earliest and highest expressed gene was a pathogen receptor, .

Citing Articles

Expression of Genes Involved in Anthracnose Resistance in Chili () 'PBC80'-Derived Recombinant Inbred Lines.

Kethom W, Taylor P, Mongkolporn O Pathogens. 2023; 12(11).

PMID: 38003772 PMC: 10675817. DOI: 10.3390/pathogens12111306.

References

Faulkner C, Petutschnig E, Benitez-Alfonso Y, Beck M, Robatzek S, Lipka V . LYM2-dependent chitin perception limits molecular flux via plasmodesmata. Proc Natl Acad Sci U S A. 2013; 110(22):9166-70. PMC: 3670346. DOI: 10.1073/pnas.1203458110. View

Ngou B, Ahn H, Ding P, Jones J . Mutual potentiation of plant immunity by cell-surface and intracellular receptors. Nature. 2021; 592(7852):110-115. DOI: 10.1038/s41586-021-03315-7. View

Savatin D, Gramegna G, Modesti V, Cervone F . Wounding in the plant tissue: the defense of a dangerous passage. Front Plant Sci. 2014; 5:470. PMC: 4165286. DOI: 10.3389/fpls.2014.00470. View

Muller M, Munne-Bosch S . Ethylene Response Factors: A Key Regulatory Hub in Hormone and Stress Signaling. Plant Physiol. 2015; 169(1):32-41. PMC: 4577411. DOI: 10.1104/pp.15.00677. View

Pitsili E, Phukan U, Coll N . Cell Death in Plant Immunity. Cold Spring Harb Perspect Biol. 2019; 12(6). PMC: 7263082. DOI: 10.1101/cshperspect.a036483. View

Son S, Kim S, Lee K, Oh J, Choi I, Do J . Identification of the NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose. Int J Mol Sci. 2021; 22(22). PMC: 8620258. DOI: 10.3390/ijms222212612. View

Hu S, Li J, Dhar N, Li J, Chen J, Jian W . Lysin Motif (LysM) Proteins: Interlinking Manipulation of Plant Immunity and Fungi. Int J Mol Sci. 2021; 22(6). PMC: 8003243. DOI: 10.3390/ijms22063114. View

Liu C, Cheng J, Zhuang Y, Ye L, Li Z, Wang Y . Polycomb repressive complex 2 attenuates ABA-induced senescence in Arabidopsis. Plant J. 2018; 97(2):368-377. DOI: 10.1111/tpj.14125. View

Kethom W, Taylor P, Mongkolporn O . Expression of Genes Involved in Anthracnose Resistance in Chili () 'PBC80'-Derived Recombinant Inbred Lines. Pathogens. 2023; 12(11). PMC: 10675817. DOI: 10.3390/pathogens12111306. View

10.

Pilet-Nayel M, Moury B, Caffier V, Montarry J, Kerlan M, Fournet S . Quantitative Resistance to Plant Pathogens in Pyramiding Strategies for Durable Crop Protection. Front Plant Sci. 2017; 8:1838. PMC: 5664368. DOI: 10.3389/fpls.2017.01838. View

11.

Doke N, Miura Y, Sanchez L, Park H, Noritake T, Yoshioka H . The oxidative burst protects plants against pathogen attack: mechanism and role as an emergency signal for plant bio-defence--a review. Gene. 1996; 179(1):45-51. DOI: 10.1016/s0378-1119(96)00423-4. View

12.

Berrocal-Lobo M, Molina A . Ethylene response factor 1 mediates Arabidopsis resistance to the soilborne fungus Fusarium oxysporum. Mol Plant Microbe Interact. 2004; 17(7):763-70. DOI: 10.1094/MPMI.2004.17.7.763. View

13.

de Lima Santos M, Resende M, Dos Santos Ciscon B, Freitas N, Pereira M, Reichel T . LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix. PLoS One. 2022; 17(2):e0258838. PMC: 8830669. DOI: 10.1371/journal.pone.0258838. View

14.

Li W, Liu Y, Wang J, He M, Zhou X, Yang C . The durably resistant rice cultivar Digu activates defence gene expression before the full maturation of Magnaporthe oryzae appressorium. Mol Plant Pathol. 2015; 17(3):354-68. PMC: 6638526. DOI: 10.1111/mpp.12286. View

15.

Isaac Kirubakaran S, Begum S, Ulaganathan K, Sakthivel N . Characterization of a new antifungal lipid transfer protein from wheat. Plant Physiol Biochem. 2008; 46(10):918-27. DOI: 10.1016/j.plaphy.2008.05.007. View

16.

Mahasuk P, Chinthaisong J, Mongkolporn O . Differential resistances to anthracnose in Capsicum baccatum as responding to two Colletotrichum pathotypes and inoculation methods. Breed Sci. 2013; 63(3):333-8. PMC: 3770561. DOI: 10.1270/jsbbs.63.333. View

17.

Lisso J, Altmann T, Mussig C . The AtNFXL1 gene encodes a NF-X1 type zinc finger protein required for growth under salt stress. FEBS Lett. 2006; 580(20):4851-6. DOI: 10.1016/j.febslet.2006.07.079. View

18.

Wu Y, Gao Y, Zhan Y, Kui H, Liu H, Yan L . Loss of the common immune coreceptor BAK1 leads to NLR-dependent cell death. Proc Natl Acad Sci U S A. 2020; 117(43):27044-27053. PMC: 7604517. DOI: 10.1073/pnas.1915339117. View

19.

Kohler C, Grossniklaus U . Epigenetic inheritance of expression states in plant development: the role of Polycomb group proteins. Curr Opin Cell Biol. 2002; 14(6):773-9. DOI: 10.1016/s0955-0674(02)00394-0. View

20.

Gilroy S, Bialasek M, Suzuki N, Gorecka M, Devireddy A, Karpinski S . ROS, Calcium, and Electric Signals: Key Mediators of Rapid Systemic Signaling in Plants. Plant Physiol. 2016; 171(3):1606-15. PMC: 4936577. DOI: 10.1104/pp.16.00434. View