High Prevalence of Multidrug-Resistant, Biofilm-Forming Virulent in Broiler Chicken Retail Points in Northeast India

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Nov 25

PMID 38002242

Authors

Govindarajan Bhuvana Priya

Kandhan Srinivas

Heiborkie Shilla

Arockiasamy Arun Prince Milton

Affiliations

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Abstract

In light of the significant public health and food safety implications associated with , this study aimed to isolate and characterize in samples obtained from broiler chicken retail points in Meghalaya, northeastern India. A total of 280 samples comprising meat, intestinal contents, water, and hand swabs were processed to detect contamination by . The isolates were subjected to toxinotyping, antimicrobial susceptibility testing, and biofilm-forming ability test. The overall occurrence of was 22.5% (17.74-27.85, 95% CI) with the highest recovery from intestine samples (31%; 22.13-41.03, 95% CI), followed by meat (23%, 15.17-32.49, 95% CI) and water samples (18%, 8.58-31.44, 95% CI). Type A was the predominant toxinotype (71.43%, 58.65-82.11, 95% CI), followed by Type A with beta2 toxin (17.46%, 9.05-29.10, 95% CI), Type C (7.94%, 2.63-17.56, 95% CI), and Type C with beta2 toxin (3.17%, 0.39-11.0, 95% CI). Nearly all (95.24%) isolates were multidrug resistant and 68.25% were biofilm formers. The predominance of multidrug-resistant and virulent Type A and Type C in retail broiler meat and intestines in the tribal-dominated northeastern region of India is of great concern from food safety and public health perspectives.

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References

Fan Y, Wang C, Wang C, Chen T, Chou C, Tsai H . Incidence and Antimicrobial Susceptibility to Clostridium perfringens in Premarket Broilers in Taiwan. Avian Dis. 2016; 60(2):444-9. DOI: 10.1637/11315-110915-Reg. View

Mahamat Abdelrahim A, Radomski N, Delannoy S, Djellal S, Le Negrate M, Hadjab K . Large-Scale Genomic Analyses and Toxinotyping of Implicated in Foodborne Outbreaks in France. Front Microbiol. 2019; 10:777. PMC: 6481350. DOI: 10.3389/fmicb.2019.00777. View

Xu W, Wang H, Chen S, Chen Y, Liu L, Wu W . Tracing Clostridium perfringens strains along the chicken production chain from farm to slaughter by multilocus sequence typing. Zoonoses Public Health. 2021; 68(5):431-442. DOI: 10.1111/zph.12831. View

Coursodon C, Glock R, Moore K, Cooper K, Songer J . TpeL-producing strains of Clostridium perfringens type A are highly virulent for broiler chicks. Anaerobe. 2011; 18(1):117-21. DOI: 10.1016/j.anaerobe.2011.10.001. View

Guran H, Oksuztepe G . Detection and typing of Clostridium perfringens from retail chicken meat parts. Lett Appl Microbiol. 2013; 57(1):77-82. DOI: 10.1111/lam.12088. View

Freedman J, Shrestha A, McClane B . Clostridium perfringens Enterotoxin: Action, Genetics, and Translational Applications. Toxins (Basel). 2016; 8(3). PMC: 4810218. DOI: 10.3390/toxins8030073. View

Thomas M, Murray R . Estimating the burden of food-borne illness in Canada. Can Commun Dis Rep. 2018; 40(14):299-302. PMC: 5864453. DOI: 10.14745/ccdr.v40i14a02. View

Charlebois A, Jacques M, Archambault M . Comparative transcriptomic analysis of Clostridium perfringens biofilms and planktonic cells. Avian Pathol. 2016; 45(5):593-601. DOI: 10.1080/03079457.2016.1189512. View

Jang Y, Kim D, Bae D, Kim S, Kim H, Moon J . Prevalence, toxin-typing, and antimicrobial susceptibility of Clostridium perfringens from retail meats in Seoul, Korea. Anaerobe. 2020; 64:102235. DOI: 10.1016/j.anaerobe.2020.102235. View

10.

Pantaleon V, Bouttier S, Soavelomandroso A, Janoir C, Candela T . Biofilms of Clostridium species. Anaerobe. 2014; 30:193-8. DOI: 10.1016/j.anaerobe.2014.09.010. View

11.

Osman K, Elhariri M . Antibiotic resistance of Clostridium perfringens isolates from broiler chickens in Egypt. Rev Sci Tech. 2014; 32(3):841-50. DOI: 10.20506/rst.32.2.2212. View

12.

Milton A, Momin A, Gandhale P, Das S, Ghatak S, Priya G . Prevalence, toxinotyping, antimicrobial susceptibility and biofilm-forming ability of Clostridium perfringens isolated from free-living rodents and shrews. Anaerobe. 2022; 77:102618. DOI: 10.1016/j.anaerobe.2022.102618. View

13.

Van Immerseel F, De Buck J, Pasmans F, Huyghebaert G, Haesebrouck F, Ducatelle R . Clostridium perfringens in poultry: an emerging threat for animal and public health. Avian Pathol. 2005; 33(6):537-49. DOI: 10.1080/03079450400013162. View

14.

Cepas V, Lopez Y, Munoz E, Rolo D, Ardanuy C, Marti S . Relationship Between Biofilm Formation and Antimicrobial Resistance in Gram-Negative Bacteria. Microb Drug Resist. 2018; 25(1):72-79. DOI: 10.1089/mdr.2018.0027. View

15.

Adams V, Han X, Lyras D, Rood J . Antibiotic resistance plasmids and mobile genetic elements of Clostridium perfringens. Plasmid. 2018; 99:32-39. DOI: 10.1016/j.plasmid.2018.07.002. View

16.

Gao X, Yang Q, Huang X, Yan Z, Zhang S, Luo R . Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathog. 2020; 147:104379. DOI: 10.1016/j.micpath.2020.104379. View

17.

Milton A, Sanjukta R, Gogoi A, Momin K, Priya G, Das S . Prevalence, molecular typing and antibiotic resistance of Clostridium perfringens in free range ducks in Northeast India. Anaerobe. 2020; 64:102242. DOI: 10.1016/j.anaerobe.2020.102242. View

18.

Koo B, Hwang E, Kim G, Park J, Oh H, Lim K . Prevalence and characterization of Clostridium perfringens isolated from feces of captive cynomolgus monkeys (Macaca fascicularis). Anaerobe. 2020; 64:102236. DOI: 10.1016/j.anaerobe.2020.102236. View

19.

Zhang S, Liu P, Wang Y, Shen Z, Wang S . Multiresistance gene cfr(C) in Clostridium perfringens of cattle origin from China. J Antimicrob Chemother. 2021; 76(12):3310-3312. DOI: 10.1093/jac/dkab339. View

20.

Hu W, Woo D, Kang Y, Koo O . Biofilm and Spore Formation of and Its Resistance to Disinfectant and Oxidative Stress. Antibiotics (Basel). 2021; 10(4). PMC: 8067515. DOI: 10.3390/antibiotics10040396. View