Inhibiting Decreases AR Expression and Inhibits Proliferation in Benign Prostate Epithelial Cells

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2023 Nov 25

PMID 38002029

Authors

Xingxing Tang

Zhifu Liu

Zheng Li

Chenchen Huang

Wei Yu

Yu Fan

Shuai Hu

Jie Jin

Affiliations

Soon will be listed here.

Abstract

(1) Background: CREB-binding protein () is a key transcriptional coactivator of androgen receptors (AR). We conducted this study to investigate the effects of on AR expression and proliferation in benign prostatic hyperplasia (BPH) prostate epithelial cells. (2) Methods: By analyzing a published data set, we found that was closely related to the gene expression of AR in prostate cells. We enrolled 20 BPH patients who underwent transurethral resection of the prostate (TURP) in Peking University First Hospital in 2022, and analyzed the expressions of and AR in BPH prostate tissues. Then, we used ICG-001 and shRNA to inhibit in prostate epithelial cells (BPH-1 cells and RWPE-1 cells), and conducted immunofluorescence, cell viability assay, flow cytometry analysis, and Western blot to analyze the effects of on AR expression and proliferation in prostate epithelial cells. We also studied the interaction between and AR through a co-immunoprecipitation assay. (3) Results: is consistent with AR in expression intensity in prostate tissues. Inhibiting decreases AR expression, and induces proliferation inhibition, apoptosis, and cell cycle arrest in BPH prostate epithelial cells. The co-immunoprecipitation assay showed that binds with AR to form transcription complexes in prostate epithelial cells. (4) Conclusions: Inhibiting decreases AR expression and inhibits proliferation in benign prostate epithelial cells. may be a potential target to affect AR expression and the proliferation of prostate epithelial cells in BPH.

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