Characterizations of the Multi-kingdom Gut Microbiota in Chinese Patients with Gouty Arthritis

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2023 Nov 24

PMID 38001408

Authors

Changming Chen

Yue Zhang

Xueming Yao

Qiulong Yan

Shenghui Li

Qin Zhong

Zhengqi Liu

Fang Tang

Can Liu

Hufan Li

Dan Zhu

Weiya Lan

Yi Ling

Daomin Lu

Hui Xu

Qiaoyi Ning

Ying Wang

Zong Jiang

Qiongyu Zhang

Guangzhao Gu

Liping Sun

Nan Wang

Guangyang Wang

Aiqin Zhang

Hayan Ullah

Wen Sun

Wukai Ma

Affiliations

Soon will be listed here.

Abstract

Objective: The gut microbial composition has been linked to metabolic and autoimmune diseases, including arthritis. However, there is a dearth of knowledge on the gut bacteriome, mycobiome, and virome in patients with gouty arthritis (GA).

Methods: We conducted a comprehensive analysis of the multi-kingdom gut microbiome of 26 GA patients and 28 healthy controls, using whole-metagenome shotgun sequencing of their stool samples.

Results: Profound alterations were observed in the gut bacteriome, mycobiome, and virome of GA patients. We identified 1,117 differentially abundant bacterial species, 23 fungal species, and 4,115 viral operational taxonomic units (vOTUs). GA-enriched bacteria included Escherichia coli_D GENOME144544, Bifidobacterium infantis GENOME095938, Blautia_A wexlerae GENOME096067, and Klebsiella pneumoniae GENOME147598, while control-enriched bacteria comprised Faecalibacterium prausnitzii_G GENOME147678, Agathobacter rectalis GENOME143712, and Bacteroides_A plebeius_A GENOME239725. GA-enriched fungi included opportunistic pathogens like Cryptococcus neoformans GCA_011057565, Candida parapsilosis GCA_000182765, and Malassezia spp., while control-enriched fungi featured several Hortaea werneckii subclades and Aspergillus fumigatus GCA_000002655. GA-enriched vOTUs mainly attributed to Siphoviridae, Myoviridae, Podoviridae, and Microviridae, whereas control-enriched vOTUs spanned 13 families, including Siphoviridae, Myoviridae, Podoviridae, Quimbyviridae, Phycodnaviridae, and crAss-like. A co-abundance network revealed intricate interactions among these multi-kingdom signatures, signifying their collective influence on the disease. Furthermore, these microbial signatures demonstrated the potential to effectively discriminate between patients and controls, highlighting their diagnostic utility.

Conclusions: This study yields crucial insights into the characteristics of the GA microbiota that may inform future mechanistic and therapeutic investigations.

Citing Articles

A population-scale analysis of 36 gut microbiome studies reveals universal species signatures for common diseases.

Sun W, Zhang Y, Guo R, Sha S, Chen C, Ullah H NPJ Biofilms Microbiomes. 2024; 10(1):96.

PMID: 39349486 PMC: 11442664. DOI: 10.1038/s41522-024-00567-9.

Causal impact of human blood metabolites and metabolic pathways on serum uric acid and gout: a mendelian randomization study.

Zhong Y, Yang C, Zhang B, Chen Y, Cai W, Wang G Front Endocrinol (Lausanne). 2024; 15:1378645.

PMID: 39027467 PMC: 11256090. DOI: 10.3389/fendo.2024.1378645.

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