Exploring Capabilities of Elemental Mass Spectrometry for Determination of Metal and Biomolecules in Extracellular Vesicles

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2023 Nov 24

PMID 37999724

Authors

Jaime Martinez-Garcia

Alicia Villa-Vazquez

Beatriz Fernandez

Hector Gonzalez-Iglesias

Rosario Pereiro

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are increasingly recognized as crucial components influencing various pathophysiological processes, such as cellular homeostasis, cancer progression, and neurological disease. However, the lack of standardized methods for EV isolation and classification, coupled with ambiguity in biochemical markers associated with EV subtypes, remains a major challenge. This Trends article highlights the most common approaches for EV isolation and characterization, along with recent applications of elemental mass spectrometry (MS) to analyse metals and biomolecules in EVs obtained from biofluids or in vitro cellular models. Considering the promising capabilities of elemental MS, the article also looks ahead to the potential analysis of EVs at the single-vesicle and single-cell levels using ICP-MS. These approaches may offer valuable insights into individual characteristics of EVs and their functions, contributing to a deeper understanding of their role in various biological processes.

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