No Fitness Cost Entailed by Type VI Secretion System Synthesis, Assembly, Contraction, or Disassembly in Enteroaggregative

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2023 Nov 16

PMID 37971272

Authors

Boris Taillefer

Julien F Giraud

Eric Cascales

Affiliations

Soon will be listed here.

Abstract

Bacteria use weapons to deliver effectors into target cells. One of these weapons, the type VI secretion system (T6SS), assembles a contractile tail acting as a spring to propel a toxin-loaded needle. Due to its size and mechanism of action, the T6SS was intuitively thought to be energetically costly. Here, using a combination of mutants and growth measurements in liquid medium, on plates, and in competition experiments, we show that the T6SS does not entail a growth cost to enteroaggregative .

Citing Articles

Analysis of the Antibiotic-Potentiating Activity, Absorption, Distribution, Metabolism, and Excretion (ADME) and the Molecular Docking Properties of Phytol Against Multi-Drug-Resistant (MDR) Strains.

Almeida-Bezerra J, Menezes S, da Costa Silva J, de Sousa S, Alves D, Alencar G Antibiotics (Basel). 2025; 13(12.

PMID: 39766561 PMC: 11672802. DOI: 10.3390/antibiotics13121171.

Selective depletion of Campylobacter jejuni via T6SS dependent functionality: an approach for improving chickens gut health.

Gupta S, Biswas P, Das B, Mondal S, Gupta P, Das D Gut Pathog. 2024; 16(1):38.

PMID: 38997758 PMC: 11245787. DOI: 10.1186/s13099-024-00628-6.

References

van Kraaij C, de Vos W, Siezen R, Kuipers O . Lantibiotics: biosynthesis, mode of action and applications. Nat Prod Rep. 1999; 16(5):575-87. DOI: 10.1039/a804531c. View

Ghoul M, Mitri S . The Ecology and Evolution of Microbial Competition. Trends Microbiol. 2016; 24(10):833-845. DOI: 10.1016/j.tim.2016.06.011. View

Granato E, Meiller-Legrand T, Foster K . The Evolution and Ecology of Bacterial Warfare. Curr Biol. 2019; 29(11):R521-R537. DOI: 10.1016/j.cub.2019.04.024. View

Taylor N, van Raaij M, Leiman P . Contractile injection systems of bacteriophages and related systems. Mol Microbiol. 2018; 108(1):6-15. DOI: 10.1111/mmi.13921. View

Douzi B, Brunet Y, Spinelli S, Lensi V, Legrand P, Blangy S . Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli. Sci Rep. 2016; 6:34405. PMC: 5048182. DOI: 10.1038/srep34405. View

Kleanthous C . Swimming against the tide: progress and challenges in our understanding of colicin translocation. Nat Rev Microbiol. 2010; 8(12):843-8. DOI: 10.1038/nrmicro2454. View

Calderon-Gonzalez R, Lee A, Lopez-Campos G, Hancock S, Sa-Pessoa J, Dumigan A . Modelling the Gastrointestinal Carriage of Klebsiella pneumoniae Infections. mBio. 2023; 14(1):e0312122. PMC: 9972987. DOI: 10.1128/mbio.03121-22. View

Wexler A, Bao Y, Whitney J, Bobay L, Xavier J, Schofield W . Human symbionts inject and neutralize antibacterial toxins to persist in the gut. Proc Natl Acad Sci U S A. 2016; 113(13):3639-44. PMC: 4822603. DOI: 10.1073/pnas.1525637113. View

Forster A, Planamente S, Manoli E, Lossi N, Freemont P, Filloux A . Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes. J Biol Chem. 2014; 289(47):33032-43. PMC: 4239648. DOI: 10.1074/jbc.M114.600510. View

10.

Chaveroche M, Ghigo J, dEnfert C . A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans. Nucleic Acids Res. 2000; 28(22):E97. PMC: 113889. DOI: 10.1093/nar/28.22.e97. View

11.

Fallingborg J . Intraluminal pH of the human gastrointestinal tract. Dan Med Bull. 1999; 46(3):183-96. View

12.

Santin Y, Doan T, Journet L, Cascales E . Cell Width Dictates Type VI Secretion Tail Length. Curr Biol. 2019; 29(21):3707-3713.e3. DOI: 10.1016/j.cub.2019.08.058. View

13.

Khan I, Bai Y, Zha L, Ullah N, Ullah H, Shah S . Mechanism of the Gut Microbiota Colonization Resistance and Enteric Pathogen Infection. Front Cell Infect Microbiol. 2022; 11:716299. PMC: 8733563. DOI: 10.3389/fcimb.2021.716299. View

14.

Logan S, Thomas J, Yan J, Baker R, Shields D, Xavier J . The type VI secretion system can modulate host intestinal mechanics to displace gut bacterial symbionts. Proc Natl Acad Sci U S A. 2018; 115(16):E3779-E3787. PMC: 5910850. DOI: 10.1073/pnas.1720133115. View

15.

Felisberto-Rodrigues C, Durand E, Aschtgen M, Blangy S, Ortiz-Lombardia M, Douzi B . Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 2011; 7(11):e1002386. PMC: 3213119. DOI: 10.1371/journal.ppat.1002386. View

16.

Milo R . What is the total number of protein molecules per cell volume? A call to rethink some published values. Bioessays. 2013; 35(12):1050-5. PMC: 3910158. DOI: 10.1002/bies.201300066. View

17.

Nguyen V, Logger L, Spinelli S, Legrand P, Huyen Pham T, Trinh T . Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol. 2017; 2:17103. DOI: 10.1038/nmicrobiol.2017.103. View

18.

Lynch M, Marinov G . The bioenergetic costs of a gene. Proc Natl Acad Sci U S A. 2015; 112(51):15690-5. PMC: 4697398. DOI: 10.1073/pnas.1514974112. View

19.

Sana T, Flaugnatti N, Lugo K, Lam L, Jacobson A, Baylot V . Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016; 113(34):E5044-51. PMC: 5003274. DOI: 10.1073/pnas.1608858113. View

20.

English G, Byron O, Cianfanelli F, Prescott A, Coulthurst S . Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK-TssFG subcomplex. Biochem J. 2014; 461(2):291-304. PMC: 4072051. DOI: 10.1042/BJ20131426. View