Site-selected in Situ Polymerization for Living Cell Surface Engineering

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Nov 10

PMID 37949881

Authors

Yihong Zhong

Lijia Xu

Chen Yang

Le Xu

Guyu Wang

Yuna Guo

Songtao Cheng

Xiao Tian

Changjiang Wang

Ran Xie

Xiaojian Wang

Lin Ding

Huangxian Ju

Affiliations

Soon will be listed here.

Abstract

The construction of polymer-based mimicry on cell surface to manipulate cell behaviors and functions offers promising prospects in the field of biotechnology and cell therapy. However, precise control of polymer grafting sites is essential to successful implementation of biomimicry and functional modulation, which has been overlooked by most current research. Herein, we report a biological site-selected, in situ controlled radical polymerization platform for living cell surface engineering. The method utilizes metabolic labeling techniques to confine the growth sites of polymers and designs a Fenton-RAFT polymerization technique with cytocompatibility. Polymers grown at different sites (glycans, proteins, lipids) have different membrane retention time and exhibit differential effects on the recognition behaviors of cellular glycans. Of particular importance is the achievement of in situ copolymerization of glycomonomers on the outermost natural glycan sites of cell membrane, building a biomimetic glycocalyx with distinct recognition properties.

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References

Su L, Feng Y, Wei K, Xu X, Liu R, Chen G . Carbohydrate-Based Macromolecular Biomaterials. Chem Rev. 2021; 121(18):10950-11029. DOI: 10.1021/acs.chemrev.0c01338. View

Facklam A, Volpatti L, Anderson D . Biomaterials for Personalized Cell Therapy. Adv Mater. 2019; 32(13):e1902005. DOI: 10.1002/adma.201902005. View

Boyce M, Carrico I, Ganguli A, Yu S, Hangauer M, Hubbard S . Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway. Proc Natl Acad Sci U S A. 2011; 108(8):3141-6. PMC: 3044403. DOI: 10.1073/pnas.1010045108. View

Rani S, Gupta U . HPMA-based polymeric conjugates in anticancer therapeutics. Drug Discov Today. 2020; 25(6):997-1012. DOI: 10.1016/j.drudis.2020.04.007. View

Reyhani A, Nothling M, Ranji-Burachaloo H, McKenzie T, Fu Q, Tan S . Blood-Catalyzed RAFT Polymerization. Angew Chem Int Ed Engl. 2018; 57(32):10288-10292. DOI: 10.1002/anie.201802544. View

Baumgartner R, Fu H, Song Z, Lin Y, Cheng J . Cooperative polymerization of α-helices induced by macromolecular architecture. Nat Chem. 2017; 9(7):614-622. DOI: 10.1038/nchem.2712. View

Cibrian D, Saiz M, de la Fuente H, Sanchez-Diaz R, Moreno-Gonzalo O, Jorge I . CD69 controls the uptake of L-tryptophan through LAT1-CD98 and AhR-dependent secretion of IL-22 in psoriasis. Nat Immunol. 2016; 17(8):985-96. PMC: 5146640. DOI: 10.1038/ni.3504. View

Zhou Z, Maxeiner K, Ng D, Weil T . Polymer Chemistry in Living Cells. Acc Chem Res. 2022; 55(20):2998-3009. PMC: 9583600. DOI: 10.1021/acs.accounts.2c00420. View

Hudak J, Canham S, Bertozzi C . Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion. Nat Chem Biol. 2013; 10(1):69-75. PMC: 3893890. DOI: 10.1038/nchembio.1388. View

10.

Delaveris C, Chiu S, Riley N, Bertozzi C . Modulation of immune cell reactivity with -binding Siglec agonists. Proc Natl Acad Sci U S A. 2021; 118(3). PMC: 7826350. DOI: 10.1073/pnas.2012408118. View

11.

Kim J, Park J . ROS-dependent caspase-9 activation in hypoxic cell death. FEBS Lett. 2003; 549(1-3):94-8. DOI: 10.1016/s0014-5793(03)00795-6. View

12.

Green J, Elisseeff J . Mimicking biological functionality with polymers for biomedical applications. Nature. 2016; 540(7633):386-394. PMC: 8186828. DOI: 10.1038/nature21005. View

13.

Guo Y, Tao J, Li Y, Feng Y, Ju H, Wang Z . Quantitative Localized Analysis Reveals Distinct Exosomal Protein-Specific Glycosignatures: Implications in Cancer Cell Subtyping, Exosome Biogenesis, and Function. J Am Chem Soc. 2020; 142(16):7404-7412. DOI: 10.1021/jacs.9b12182. View

14.

Reyhani A, McKenzie T, Ranji-Burachaloo H, Fu Q, Qiao G . Fenton-RAFT Polymerization: An "On-Demand" Chain-Growth Method. Chemistry. 2017; 23(30):7221-7226. DOI: 10.1002/chem.201701410. View

15.

Uvyn A, De Coen R, Gruijs M, Tuk C, De Vrieze J, van Egmond M . Efficient Innate Immune Killing of Cancer Cells Triggered by Cell-Surface Anchoring of Multivalent Antibody-Recruiting Polymers. Angew Chem Int Ed Engl. 2019; 58(37):12988-12993. DOI: 10.1002/anie.201905093. View

16.

Vargason A, Anselmo A, Mitragotri S . The evolution of commercial drug delivery technologies. Nat Biomed Eng. 2021; 5(9):951-967. DOI: 10.1038/s41551-021-00698-w. View

17.

Tomas R, Gibson M . Covalent cell surface recruitment of chemotherapeutic polymers enhances selectivity and activity. Chem Sci. 2021; 12(12):4557-4569. PMC: 8179505. DOI: 10.1039/d0sc06580c. View

18.

Stevens M, George J . Exploring and engineering the cell surface interface. Science. 2005; 310(5751):1135-8. DOI: 10.1126/science.1106587. View

19.

Huang M, Smith R, Trieger G, Godula K . Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells. J Am Chem Soc. 2014; 136(30):10565-8. PMC: 4121001. DOI: 10.1021/ja505012a. View

20.

Monsigny M, Sene C, OBRENOVITCH A, Roche A, Delmotte F, Boschetti E . Properties of succinylated wheat-germ agglutinin. Eur J Biochem. 1979; 98(1):39-45. DOI: 10.1111/j.1432-1033.1979.tb13157.x. View