Hepatocellular Carcinoma with Lung Metastasis Showing Hemochromatosis in an Egyptian Fruit Bat (Rousettus Aegyptiacus)

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2023 Nov 8

PMID 37940547

Authors

Nijiho Kawaguchi

Naoyuki Fuke

Phawut Nueangphuet

Apisit Pornthummawat

Ahmad Massoud Niazi

Uda Zahli Izzati

Takuya Hirai

Ryoji Yamaguchi

Affiliations

Soon will be listed here.

Abstract

After an Egyptian fruit bat (Rousettus aegyptiacus) in a zoo became emaciated and died, a necropsy revealed multiple nodules on the liver and lung surfaces. Microscopy revealed that the liver nodules consisted of neoplastic hepatocytes and showed metastasis in the lung lobes. Most of the neoplastic cells in the liver and lung showed positive labeling for HepPar-1, cytokeratin 19, glypican-3, and Ki-67. Hepatocellular degeneration and necrosis were diffuse in the liver parenchyma. Berlin blue staining revealed large amounts of iron in normal and neoplastic cells. Based on these pieces of evidence, this case was diagnosed as hepatocellular carcinoma with hemochromatosis. This is believed to be the first report of hepatocellular carcinoma in an Egyptian fruit bat that has been immunophenotypically examined in detail by pathological examination.

References

Olds J, Burrough E, Fales-Williams A, Lehmkuhl A, Madson D, Patterson A . RETROSPECTIVE EVALUATION OF CASES OF NEOPLASIA IN A CAPTIVE POPULATION OF EGYPTIAN FRUIT BATS (ROUSETTUS AEGYPTIACUS). J Zoo Wildl Med. 2015; 46(2):325-32. DOI: 10.1638/2014-0069R2.1. View

Asare G, Mossanda K, Kew M, Paterson A, Kahler-Venter C, Siziba K . Hepatocellular carcinoma caused by iron overload: a possible mechanism of direct hepatocarcinogenicity. Toxicology. 2005; 219(1-3):41-52. DOI: 10.1016/j.tox.2005.11.006. View

Bae I, Pakhrin B, Jee H, Shin N, Kim D . Hepatocellular adenoma in a Eurasian otter (Lutra lutra). J Vet Sci. 2007; 8(1):103-5. PMC: 2872691. DOI: 10.4142/jvs.2007.8.1.103. View

Marrow J, Basu P, Walsh T, Siegal-Willott J . Hepatocellular carcinoma in captive slender tailed meerkats (Suricata suricatta): 5 cases. J Zoo Wildl Med. 2014; 45(1):134-42. DOI: 10.1638/2013-0142R.1. View

Strohmeyer G, Niederau C, Stremmel W . Survival and causes of death in hemochromatosis. Observations in 163 patients. Ann N Y Acad Sci. 1988; 526:245-57. DOI: 10.1111/j.1749-6632.1988.tb55510.x. View

Fracanzani A, Conte D, Fraquelli M, Taioli E, Mattioli M, Losco A . Increased cancer risk in a cohort of 230 patients with hereditary hemochromatosis in comparison to matched control patients with non-iron-related chronic liver disease. Hepatology. 2001; 33(3):647-51. DOI: 10.1053/jhep.2001.22506. View

van Sprundel R, van den Ingh T, Schotanus B, van Wolferen M, Penning L, Rothuizen J . Cellular characteristics of keratin 19-positive canine hepatocellular tumours explain its aggressive behaviour. Vet Rec Open. 2017; 4(1):e000212. PMC: 5663258. DOI: 10.1136/vetreco-2016-000212. View

Siegal-Willott J, Heard D, Sliess N, Naydan D, Roberts J . Microchip-associated leiomyosarcoma in an Egyptian fruit bat (Rousettus aegyptiacus). J Zoo Wildl Med. 2007; 38(2):352-6. DOI: 10.1638/1042-7260(2007)038[0352:MLIAEF]2.0.CO;2. View

Stasiak I, Smith D, Ganz T, Crawshaw G, Hammermueller J, Bienzle D . Iron storage disease (hemochromatosis) and hepcidin response to iron load in two species of pteropodid fruit bats relative to the common vampire bat. J Comp Physiol B. 2018; 188(4):683-694. DOI: 10.1007/s00360-018-1155-4. View

10.

Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M . Immunoassay for human serum hepcidin. Blood. 2008; 112(10):4292-7. DOI: 10.1182/blood-2008-02-139915. View

11.

Bradford C, Jennings R, Ramos-Vara J . Gastrointestinal leiomyosarcoma in an Egyptian fruit bat (Rousettus aegyptiacus). J Vet Diagn Invest. 2010; 22(3):462-5. DOI: 10.1177/104063871002200324. View

12.

Chege S, Vissiennon T, Cavero T, Kinne J, Toosy A . A RARE CASE OF HEPATOCELLULAR CARCINOMA IN THE ARABIAN SAND CAT (FELIS MARGARITA HARRISONI). J Zoo Wildl Med. 2015; 46(4):699-703. DOI: 10.1638/2015-0062.1. View

13.

Durnez A, Verslype C, Nevens F, Fevery J, Aerts R, Pirenne J . The clinicopathological and prognostic relevance of cytokeratin 7 and 19 expression in hepatocellular carcinoma. A possible progenitor cell origin. Histopathology. 2006; 49(2):138-51. DOI: 10.1111/j.1365-2559.2006.02468.x. View

14.

Paganoni R, Lechel A, Vujic Spasic M . Iron at the Interface of Hepatocellular Carcinoma. Int J Mol Sci. 2021; 22(8). PMC: 8071427. DOI: 10.3390/ijms22084097. View

15.

Blanc J, de Ledinghen V, Bernard P, de Verneuil H, Winnock M, Le Bail B . Increased incidence of HFE C282Y mutations in patients with iron overload and hepatocellular carcinoma developed in non-cirrhotic liver. J Hepatol. 2000; 32(5):805-11. DOI: 10.1016/s0168-8278(00)80250-0. View

16.

Uenishi T, Kubo S, Yamamoto T, Shuto T, Ogawa M, Tanaka H . Cytokeratin 19 expression in hepatocellular carcinoma predicts early postoperative recurrence. Cancer Sci. 2003; 94(10):851-7. PMC: 11160230. DOI: 10.1111/j.1349-7006.2003.tb01366.x. View

17.

Maeda T, Adachi E, Kajiyama K, Takenaka K, Sugimachi K, Tsuneyoshi M . Proliferative activity in intrahepatic metastasis of hepatocellular carcinoma. J Gastroenterol Hepatol. 1996; 11(8):752-7. DOI: 10.1111/j.1440-1746.1996.tb00326.x. View

18.

Shafizadeh N, Ferrell L, Kakar S . Utility and limitations of glypican-3 expression for the diagnosis of hepatocellular carcinoma at both ends of the differentiation spectrum. Mod Pathol. 2008; 21(8):1011-8. DOI: 10.1038/modpathol.2008.85. View

19.

Leone A, Crawshaw G, Garner M, Frasca Jr S, Stasiak I, Rose K . A RETROSPECTIVE STUDY OF THE LESIONS ASSOCIATED WITH IRON STORAGE DISEASE IN CAPTIVE EGYPTIAN FRUIT BATS (ROUSETTUS AEGYPTIACUS). J Zoo Wildl Med. 2016; 47(1):45-55. DOI: 10.1638/2015-0224.1. View

20.

Almeida J, Iriabho E, Gorrepati V, Wilkinson S, Gruneberg A, Robbins D . ImageJS: Personalized, participated, pervasive, and reproducible image bioinformatics in the web browser. J Pathol Inform. 2012; 3:25. PMC: 3424663. DOI: 10.4103/2153-3539.98813. View