Cellular Characteristics of Keratin 19-positive Canine Hepatocellular Tumours Explain Its Aggressive Behaviour

Overview

Journal Vet Rec Open

Publisher Wiley

Specialty Veterinary Medicine

Date 2017 Nov 10

PMID 29118993

Citations 3

Authors

Renee G van Sprundel

Ted Sgam van den Ingh

Baukje A Schotanus

Monique E van Wolferen

Louis C Penning

Jan Rothuizen

Bart Spee

Affiliations

Soon will be listed here.

Abstract

The expression of the hepatic progenitor cell marker keratin 19 (K19) in canine hepatocellular carcinomas is linked with a poor prognosis. To better understand this aggressive behaviour, K19-positive hepatocellular carcinomas (n=5) and K19-negative hepatocellular adenomas (n=6) were immunohistochemically stained for proteins involved in malignant tumour development. The K19-positive carcinomas showed marked positivity for platelet-derived growth factor receptor alpha polypeptide (PDGFRα), laminin, integrin beta-1/CD29, B-cell-specific Moloney murine leukaemia virus Integration site 1, glypican-3 (GPC-3) and prominin-1/CD133, in contrast with K19-negative hepatocellular adenomas. Conversely, neurofibromatosis type 2 was highly expressed in the hepatocellular adenomas in contrast with the hepatocellular carcinomas. This expression pattern is clearly in line with the observed aggressive behaviour. The presence of the malignancy markers PDGFRα and GPC-3 might make it possible to develop specific strategies to intervene in tumour growth and to devise novel serological tests and personalised treatment methods for canine hepatocellular carcinomas.

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