Cryo-EM Structure of a Shigella Podophage Reveals a Hybrid Tail and Novel Decoration Proteins

Overview

Journal Structure

Publisher Cell Press

Specialties Biochemistry
Biotechnology
Cell Biology
Molecular Biology

Date 2023 Nov 1

PMID 37909043

Authors

Sundharraman Subramanian

Silje M Bergland Drarvik

Kendal R Tinney

Kristin N Parent

Affiliations

Soon will be listed here.

Abstract

There is a paucity of high-resolution structures of phages infecting Shigella, a human pathogen and a serious threat to global health. HRP29 is a Shigella podophage belonging to the Autographivirinae family, and has very low sequence identity to other known phages. Here, we resolved the structure of the entire HRP29 virion by cryo-EM. Phage HRP29 has a highly unusual tail that is a fusion of a T7-like tail tube and P22-like tailspikes mediated by interactions from a novel tailspike adaptor protein. Understanding phage tail structures is critical as they mediate hosts interactions. Furthermore, we show that the HRP29 capsid is stabilized by two novel, and essential decoration proteins, gp47 and gp48. Only one high resolution structure is currently available for Shigella podophages. The presence of a hybrid tail and an adapter protein suggests that it may be a product of horizontal gene transfer, and may be prevalent in other phages.

Citing Articles

Moo19 and B2: Structures of podophages with = 9 geometry and tailspikes with esterase activity.

Subramanian S, Bergland Drarvik S, Tinney K, Doore S, Parent K Sci Adv. 2024; 10(51):eadt0022.

PMID: 39693418 PMC: 11654681. DOI: 10.1126/sciadv.adt0022.

Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146.

Pas C, Fieseler L, Pothier J, Briers Y Sci Rep. 2024; 14(1):26050.

PMID: 39472643 PMC: 11522302. DOI: 10.1038/s41598-024-77463-x.

Ejectosome of bacteriophage ΦM1.

Eruera A, Hodgkinson-Bean J, Rutter G, Hills F, Kumaran R, Crowe A PNAS Nexus. 2024; 3(9):pgae416.

PMID: 39351541 PMC: 11440229. DOI: 10.1093/pnasnexus/pgae416.

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