Moo19 and B2: Structures of Podophages with = 9 Geometry and Tailspikes with Esterase Activity

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2024 Dec 18

PMID 39693418

Authors

Sundharraman Subramanian

Silje M Bergland Drarvik

Kendal R Tinney

Sarah M Doore

Kristin N Parent

Affiliations

Soon will be listed here.

Abstract

Podophages are, by far, the least well studied of all the bacteriophages. Despite being classified together due to their short, noncontractile tails, there is a huge amount of diversity among members of this group. Of the podophages, the N4-like family is the least well studied structurally and is quite divergent from well-characterized podophages such as T7 and P22. In this work, we isolate and fully characterize two members of the family by cryo-electron microscopy, genetics, and biochemistry. We describe the capsid features of Moo19 and B2, including a decoration protein. In addition, we have fully modeled the tail machinery for both phages and identify proteins with esterase activity. Genetic knockouts of the host reveal factors specific for host attachment including key modifications to the O-antigen on the lipopolysaccharide. Moo19 and B2 are both members, yet some distinct differences in the genome and structure place them into distinct clades.

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