Exoribonuclease RNase R Protects Antarctic Lz4W from DNA Damage and Oxidative Stress

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2023 Oct 31

PMID 37905926

Authors

Pragya Mittal

Rashmi Sipani

Apuratha Pandiyan

Shaheen Sulthana

Anurag K Sinha

Ashaq Hussain

Malay K Ray

Theetha L Pavankumar

Affiliations

Soon will be listed here.

Abstract

Bacterial exoribonucleases play a crucial role in RNA maturation, degradation, quality control, and turnover. In this study, we have uncovered a previously unknown role of 3'-5' exoribonuclease RNase R of Lz4W in DNA damage and oxidative stress response. Here, we show that neither the exoribonuclease function of RNase R nor its association with the RNA degradosome complex is essential for this function. Interestingly, in Lz4W, hydrolytic RNase R exhibits physiological roles similar to phosphorolytic 3'-5' exoribonuclease PNPase of . Our data suggest that during the course of evolution, mesophilic and psychrotrophic have apparently swapped these exoribonucleases to adapt to their respective environmental growth conditions.

Citing Articles

A type II toxin-antitoxin system is responsible for the cell death at low temperature in Pseudomonas syringae Lz4W lacking RNase R.

Mittal P, Sinha A, Pandiyan A, Kumari L, Ray M, Pavankumar T J Biol Chem. 2024; 300(8):107600.

PMID: 39059490 PMC: 11375266. DOI: 10.1016/j.jbc.2024.107600.

RNase R vs. PNPase: selecting the best-suited exoribonuclease for environmental adaptation.

Pavankumar T Extremophiles. 2024; 28(3):35.

PMID: 39052080 DOI: 10.1007/s00792-024-01350-6.

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