Endotoxin Lethality and Tolerance in Mice: Analysis with the B-lymphocyte-defective CBA/N Strain

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1979 Apr 1

PMID 378841

Citations 4

Authors

N M Zaldivar

I SCHER

Affiliations

Soon will be listed here.

Abstract

Immune-defective and immunologically normal F1 mice derived from the CBA/N strain were used to study the influence of anti-endotoxin antibody on the lethal effects of endotoxin. Immune-defective F1 male mice were unable to make specific responses to purified preparations of E. coli O111:B4 endotoxin, whereas their immunologically normal F1 female littermates made excellent responses. The ability to form antibody to lipopolysaccharide (LPS) in these F1 mice did not influence either their natural resistance to endotoxin challenge or the effects of pretreatment with sublethal amounts of endotoxin on subsequent challenge with higher normally lethal doses. Furthermore, transfer of sera with high titers of anti-LPS antibody to mice prior to challenge with LPS failed to protect. Thus, anti-LPS antibody does not appear to play a critical role in protection of immune-defective (CBA/N X DBA/2) F1 male mice to the lethal effects of endotoxin or to the protective effects of a single sublethal dose of endotoxin on subsequent endotoxin challenge.

Citing Articles

Relationship between Endotoxin Content in Vaccine Preclinical Formulations and Animal Welfare: An Extensive Study on Historical Data to Set an Informed Threshold.

Baffetta F, Cecchi R, Guerrini E, Mangiavacchi S, Sorrentino G, Stranges D Vaccines (Basel). 2024; 12(7).

PMID: 39066452 PMC: 11281343. DOI: 10.3390/vaccines12070815.

Enhanced phagocytosis and bactericidal activity of hepatic reticuloendothelial system during endotoxin tolerance.

Ruggiero G, Andreana A, Utili R, Galante D Infect Immun. 1980; 27(3):798-803.

PMID: 6991430 PMC: 550842. DOI: 10.1128/iai.27.3.798-803.1980.

Antibody response of immunodeficient (xid) CBA/N mice to Escherichia coli 0113 lipopolysaccharide, a thymus-independent antigen.

Hiernaux J, Jones J, Rudbach J, Rollwagen F, Baker P J Exp Med. 1983; 157(4):1197-207.

PMID: 6187886 PMC: 2186980. DOI: 10.1084/jem.157.4.1197.

Enhanced endotoxin clearance in reversed Eck fistula rats during a tolerant stage.

Yamaguchi Y, Mori K, Gans H, Akagi M Jpn J Surg. 1986; 16(3):209-17.

PMID: 3525929 DOI: 10.1007/BF02471095.

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