Simultaneous Extraction and Detection of Peptides, Steroids, and Proteins in Small Tissue Samples

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2023 Oct 25

PMID 37876537

Authors

Chunyu Lu

Di Peng

W C K Udeesha Erandani

Kimberly Mitchell

Christopher J Martyniuk

Vance L Trudeau

Affiliations

Soon will be listed here.

Abstract

The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.

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