Quantitative Peptidomics of Mouse Brain After Infection With Cyst-Forming

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2021 Aug 9

PMID 34367142

Citations 7

Authors

Chun-Xue Zhou

Min Gao

Bing Han

Hua Cong

Xing-Quan Zhu

Huai-Yu Zhou

Affiliations

Soon will be listed here.

Abstract

is an obligate intracellular parasite capable of establishing persistent infection within the host brain and inducing severe neuropathology. Peptides are important native molecules responsible for a wide range of biological functions within the central nervous system. However, peptidome profiling in host brain during infection has never been investigated. Using a label-free peptidomics approach (LC-MS/MS), we identified a total of 2,735 endogenous peptides from acutely infected, chronically infected and control brain samples following infection. Quantitative analysis revealed 478 and 344 significantly differentially expressed peptides (DEPs) in the acute and chronic infection stages, respectively. Functional analysis of DEPs by Gene Ontology suggested these DEPs mainly originated from cell part and took part in cellular process. We also identified three novel neuropeptides derived from the precursor protein cholecystokinin. These results demonstrated the usefulness of quantitative peptidomics in determining bioactive peptides and elucidating their functions in the regulation of behavior modification during infection.

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