De Novo Transcriptome Assembly of An Antarctic Nematode for the Study of Thermal Adaptation in Marine Parasites

Overview

Journal Sci Data

Specialty Science

Date 2023 Oct 19

PMID 37857654

Authors

Marialetizia Palomba

Pietro Libro

Jessica Di Martino

Xavier Roca-Gerones

Armando Macali

Tiziana Castrignano

Daniele Canestrelli

Simonetta Mattiucci

Affiliations

Soon will be listed here.

Abstract

Understanding the genomic underpinnings of thermal adaptation is a hot topic in eco-evolutionary studies of parasites. Marine heteroxenous parasites have complex life cycles encompassing a free-living larval stage, an ectothermic intermediate host and a homeothermic definitive host, thus representing compelling systems for the study of thermal adaptation. The Antarctic anisakid Contracaecum osculatum sp. D is a marine parasite able to survive and thrive both at very cold and warm temperatures within the environment and its hosts. Here, a de novo transcriptome of C. osculatum sp. D was generated for the first time, by performing RNA-Seq experiments on a set of individuals exposed to temperatures experienced by the nematode during its life cycle. The analysis generated 425,954,724 reads, which were assembled and then annotated. The high-quality assembly was validated, achieving over 88% mapping against the transcriptome. The transcriptome of this parasite will represent a valuable genomic resource for future studies aimed at disentangling the genomic architecture of thermal tolerance and metabolic pathways related to temperature stress.

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