Temporal Stability of Parasite Distribution and Genetic Variability Values of Contracaecum Osculatum Sp. D and C. osculatum Sp. E (Nematoda: Anisakidae) from Fish of the Ross Sea (Antarctica)

Overview

Journal Int J Parasitol Parasites Wildl

Date 2016 Jan 15

PMID 26767164

Citations 9

Authors

Simonetta Mattiucci

Paolo Cipriani

Michela Paoletti

Valentina Nardi

Mario Santoro

Bruno Bellisario

Giuseppe Nascetti

Affiliations

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Abstract

The Ross Sea, Eastern Antarctica, is considered a "pristine ecosystem" and a biodiversity "hotspot" scarcely impacted by humans. The sibling species Contracaecum osculatum sp. D and C. osculatum sp. E are anisakid parasites embedded in the natural Antarctic marine ecosystem. Aims of this study were to: identify the larvae of C. osculatum (s.l.) recovered in fish hosts during the XXVII Italian Expedition to Antarctica (2011-2012); perform a comparative analysis of the contemporary parasitic load and genetic variability estimates of C. osculatum sp. D and C. osculatum sp. E with respect to samples collected during the expedition of 1993-1994; to provide ecological data on these parasites. 200 fish specimens (Chionodraco hamatus, Trematomus bernacchii, Trematomus hansoni, Trematomus newnesi) were analysed for Contracaecum sp. larvae, identified at species level by allozyme diagnostic markers and sequences analysis of the mtDNA cox2 gene. Statistically significant differences were found between the occurrence of C. osculatum sp. D and C. osculatum sp. E in different fish species. C. osculatum sp. E was more prevalent in T. bernacchii; while, a higher percentage of C. osculatum sp. D occurred in Ch. hamatus and T. hansoni. The two species also showed differences in the host infection site: C. osculatum sp. D showed higher percentage of infection in the fish liver. High genetic variability values at both nuclear and mitochondrial level were found in the two species in both sampling periods. The parasitic infection levels by C. osculatum sp. D and sp. E and their estimates of genetic variability showed no statistically significant variation over a temporal scale (2012 versus 1994). This suggests that the low habitat disturbance of the Antarctic region permits the maintenance of stable ecosystem trophic webs, which contributes to the maintenance of a large populations of anisakid nematodes with high genetic variability.

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References

Zhu X, DAmelio S, Paggi L, Gasser R . Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitol Res. 2000; 86(8):677-83. DOI: 10.1007/pl00008551. View

Mattiucci S, Nascetti G, Clanchi R, Paggi L, Arduino P, Margolis L . Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). J Parasitol. 1997; 83(3):401-16. View

Koie M, Fagerholm H . The life cycle of Contracaecum osculatum (Rudolphi, 1802) sensu stricto (Nematoda, Ascaridoidea, Anisakidae) in view of experimental infections. Parasitol Res. 1995; 81(6):481-9. DOI: 10.1007/BF00931790. View

Reeder T . A phylogeny of the Australian Sphenomorphus group (Scincidae: Squamata) and the phylogenetic placement of the crocodile skinks (Tribolonotus): Bayesian approaches to assessing congruence and obtaining confidence in maximum likelihood inferred.... Mol Phylogenet Evol. 2003; 27(3):384-97. DOI: 10.1016/s1055-7903(02)00448-7. View

Ronquist F, Teslenko M, van der Mark P, Ayres D, Darling A, Hohna S . MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012; 61(3):539-42. PMC: 3329765. DOI: 10.1093/sysbio/sys029. View

Mattiucci S, Nascetti G . Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Adv Parasitol. 2008; 66:47-148. DOI: 10.1016/S0065-308X(08)00202-9. View

Mattiucci S, Cipriani P, Webb S, Paoletti M, Marcer F, Bellisario B . Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). J Parasitol. 2013; 100(2):199-214. DOI: 10.1645/12-120.1. View

Posada D . jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008; 25(7):1253-6. DOI: 10.1093/molbev/msn083. View

Valentini A, Mattiucci S, Bondanelli P, Webb S, Mignucci-Giannone A, Colom-Llavina M . Genetic relationships among Anisakis species (Nematoda: Anisakidae) inferred from mitochondrial cox2 sequences, and comparison with allozyme data. J Parasitol. 2006; 92(1):156-66. DOI: 10.1645/GE-3504.1. View

10.

Nadler S, DAmelio S, Dailey M, Paggi L, Siu S, Sakanari J . Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals. J Parasitol. 2006; 91(6):1413-29. DOI: 10.1645/GE-522R.1. View

11.

Hoberg E, Brooks D . Evolution in action: climate change, biodiversity dynamics and emerging infectious disease. Philos Trans R Soc Lond B Biol Sci. 2015; 370(1665). PMC: 4342959. DOI: 10.1098/rstb.2013.0553. View

12.

Garbin L, Mattiucci S, Paoletti M, Gonzalez-Acuna D, Nascetti G . Genetic and morphological evidences for the existence of a new species of Contracaecum (Nematoda: Anisakidae) parasite of Phalacrocorax brasilianus (Gmelin) from Chile and its genetic relationships with congeners from fish-eating birds. J Parasitol. 2011; 97(3):476-92. DOI: 10.1645/GE-2450.1. View

13.

Mattiucci S, Nascetti G . Genetic diversity and infection levels of anisakid nematodes parasitic in fish and marine mammals from Boreal and Austral hemispheres. Vet Parasitol. 2007; 148(1):43-57. DOI: 10.1016/j.vetpar.2007.05.009. View

14.

Posada D, Buckley T . Model selection and model averaging in phylogenetics: advantages of akaike information criterion and bayesian approaches over likelihood ratio tests. Syst Biol. 2004; 53(5):793-808. DOI: 10.1080/10635150490522304. View

15.

Nadler S, Hudspeth D . Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution. J Parasitol. 2000; 86(2):380-93. DOI: 10.1645/0022-3395(2000)086[0380:POTANA]2.0.CO;2. View

16.

Rutschmann S, Matschiner M, Damerau M, Muschick M, Lehmann M, Hanel R . Parallel ecological diversification in Antarctic notothenioid fishes as evidence for adaptive radiation. Mol Ecol. 2011; 20(22):4707-21. DOI: 10.1111/j.1365-294X.2011.05279.x. View

17.

Shamsi S . Recent advances in our knowledge of Australian anisakid nematodes. Int J Parasitol Parasites Wildl. 2014; 3(2):178-87. PMC: 4145145. DOI: 10.1016/j.ijppaw.2014.04.001. View

18.

Nascetti G, Cianchi R, Mattiucci S, DAmelio S, Orecchia P, Paggi L . Three sibling species within Contracaecum osculatum (Nematoda, Ascaridida, Ascaridoidea) from the Atlantic Arctic-Boreal region: reproductive isolation and host preferences. Int J Parasitol. 1993; 23(1):105-20. DOI: 10.1016/0020-7519(93)90103-6. View

19.

Hu M, DAmelio S, Zhu X, Paggi L, Gasser R . Mutation scanning for sequence variation in three mitochondrial DNA regions for members of the Contracaecum osculatum (Nematoda: Ascaridoidea) complex. Electrophoresis. 2001; 22(6):1069-75. DOI: 10.1002/1522-2683()22:6<1069::AID-ELPS1069>3.0.CO;2-T. View

20.

Blazekovic K, Lepen Pleic I, duras M, Gomercic T, Mladineo I . Three Anisakis spp. isolated from toothed whales stranded along the eastern Adriatic Sea coast. Int J Parasitol. 2014; 45(1):17-31. DOI: 10.1016/j.ijpara.2014.07.012. View