Xanthones Isolated from Reduced Oxidative Stress in Periodontal Ligament Stem Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Oct 14

PMID 37834121

Authors

Nisarat Ruangsawasdi

Nawong Boonnak

Chareerut Pruksaniyom

Pirasut Rodanant

Affiliations

Soon will be listed here.

Abstract

Xanthone compounds from () have demonstrated antioxidant effects and potency in treating many inflammatory diseases. However, the efficiency of the three xanthone extracts isolated from the young fruit of this plant, i.e., two geranyloxy xanthones (F6, F8) and one 1,3,7-hydroxy xanthone (F137), as antioxidants and therapeutics for periodontal disease has not been evaluated. The aim of this study was to investigate the antioxidant effects of three xanthones isolated from on periodontal ligament stem cells (PDLSCs) and their osteogenic differentiation. The antioxidant activity of the aqueous extracts was determined using a DPPH assay, and their cytotoxicity was evaluated using an MTT assay. HO was used to induce intracellular stress, and the scavenging effect of the isolated compounds against reactive oxygen species (ROS) was analyzed with a fluorescence assay. The expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was evaluated, and the effects of the three compounds on PDLSCs osteogenic differentiation were investigated. The isolated compounds reduced both extracellular and intracellular ROS in a dose-dependent manner and induced the expression of Nrf2 and HO-1 in PDLSCs. Under redox conditions, these compounds potentiated PDLSCs osteogenic differentiation. Our study demonstrated that the hydroxy xanthones from had antioxidant effects on the Nrf2/HO-1 pathway and might be effective therapeutic substrates for damage prevention and the regeneration of damaged periodontal tissues in periodontitis patients.

Citing Articles

Ubiquitin-Proteasome System in Periodontitis: Mechanisms and Clinical Implications.

Ma Y, Jia R, Chen S, Ma J, Yin L, Pan X Cell Prolif. 2024; 58(3):e13781.

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Heme oxygenase-1: potential therapeutic targets for periodontitis.

Lv W, Hu S, Yang F, Lin D, Zou H, Zhang W PeerJ. 2024; 12:e18237.

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