Investigation of the Effect of Vitamin K1 Prophylaxis on Newborn Screenings Tests in Newborns

Overview

Journal J Med Biochem

Specialty Biochemistry

Date 2023 Oct 10

PMID 37814616

Authors

Murat Caglayan

Ataman Gonel

Cuneyt Tayman

Ufuk Cakir

Ismail Koyuncu

Ebru Temiz

Yasemin Sert

Affiliations

Soon will be listed here.

Abstract

Background: Routine screening for hereditary disorders in newborns includes screening for treatable metabolic and endocrine disorders, such as biotidinase deficiency, galactosemia, maple syrup urine disease, hypothyroidism, and cystic fibrosis. Incorrect test results may be encountered due to the use of vitamin K1. To investigate the interference effect of vitamin K1 on neonatal screening tests and to raise awareness of erroneous measurements.

Methods: Heel blood samples were taken from 25 newborns born in a neonatal intensive care unit. Dry blood C0, C2, C3, C4, C4DC, C5:1, C5OH, C5DC, C6, C6DC, C8, C8:1, C8DC, C10, C10:1, C10DC, C12, C14, C14:1, C14:2, C16, C16:1, C18, C18:1, C18:2, C18:OH, methylglutaryl, valine, leucine/isoleucine, methionine, phenylalanine, argininosuccinic acid, aspartate, alanine, arginine, citrulline, glycine, ornithine, and glutamate tests were studied using the tandem mass spectrometry (MS) method. The results of the heel blood samples obtained before and after the application of vitamin K1 (Phyto menadione) were compared.

Results: In two studies conducted with in vitro and in vivo tests, C0, C2, C3, C4, C4DC, C5, C5OH, C6, C8, C10, C10:1, C14, C16, C16:1, C18, C18:1, methylglutaryl, phenylalanine, argininosuccinic acid, tyrosine, aspartate, arginine, citrulline, glycine, and glutamine were all significantly elevated (p < 0.05).

Conclusions: Heel blood samples may yield false results due to vitamin K1 administration. In the case of doubtful results, a new sample should be taken and the measurement should be repeated.

References

Samarasinghe S, Meah F, Singh V, Basit A, Emanuele N, Emanuele M . BIOTIN INTERFERENCE WITH ROUTINE CLINICAL IMMUNOASSAYS: UNDERSTAND THE CAUSES AND MITIGATE THE RISKS. Endocr Pract. 2017; 23(8):989-998. DOI: 10.4158/EP171761.RA. View

Pehlivan V, Gonel A, Pehlivan B, Koyuncu I . False Laboratory Test Result Through Colistin Interference in an Intensive Care Patient: Case Report. Comb Chem High Throughput Screen. 2020; 23(8):827-831. DOI: 10.2174/1386207323666200514072705. View

Cunniff J, Vouros P . False positives and the detection of cyclodextrin inclusion complexes by electrospray mass spectrometry. J Am Soc Mass Spectrom. 2013; 6(5):437-47. DOI: 10.1016/1044-0305(95)00053-G. View

Chun K . Biotin Interference in Diagnostic Tests. Clin Chem. 2017; 63(2):619-620. DOI: 10.1373/clinchem.2016.267286. View

Souverain S, Rudaz S, Veuthey J . Matrix effect in LC-ESI-MS and LC-APCI-MS with off-line and on-line extraction procedures. J Chromatogr A. 2004; 1058(1-2):61-6. View

Bowen R, Benavides R, Colon-Franco J, Katzman B, Muthukumar A, Sadrzadeh H . Best practices in mitigating the risk of biotin interference with laboratory testing. Clin Biochem. 2019; 74:1-11. DOI: 10.1016/j.clinbiochem.2019.08.012. View

Ucles S, Lozano A, Sosa A, Parrilla Vazquez P, Valverde A, Fernandez-Alba A . Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry. Talanta. 2017; 174:72-81. DOI: 10.1016/j.talanta.2017.05.068. View

Piketty M, Prie D, Sedel F, Bernard D, Hercend C, Chanson P . High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference. Clin Chem Lab Med. 2017; 55(6):817-825. DOI: 10.1515/cclm-2016-1183. View

Simpson J, Zhang Q, Ozaeta P, Aboleneen H . A specific method for the measurement of cyclosporin A in human whole blood by liquid chromatography-tandem mass spectrometry. Ther Drug Monit. 1998; 20(3):294-300. DOI: 10.1097/00007691-199806000-00009. View

10.

la Marca G, Malvagia S, Pasquini E, Innocenti M, Fernandez M, Donati M . The inclusion of succinylacetone as marker for tyrosinemia type I in expanded newborn screening programs. Rapid Commun Mass Spectrom. 2008; 22(6):812-8. DOI: 10.1002/rcm.3428. View

11.

Millington D, Kodo N, Norwood D, Roe C . Tandem mass spectrometry: a new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inherit Metab Dis. 1990; 13(3):321-4. DOI: 10.1007/BF01799385. View

12.

Colon P, Greene D . Biotin Interference in Clinical Immunoassays. J Appl Lab Med. 2021; 2(6):941-951. DOI: 10.1373/jalm.2017.024257. View

13.

Gonel A, Kirhan I, Koyuncu I, Bayraktar N, Ercan Karadag M, Karadag M . The Role of Interferences in the Increasing Incidence of Vitamin D Deficiency. Endocr Metab Immune Disord Drug Targets. 2020; 20(8):1303-1308. DOI: 10.2174/1871530320666200604160208. View

14.

Azzari C, la Marca G, Resti M . Neonatal screening for severe combined immunodeficiency caused by an adenosine deaminase defect: a reliable and inexpensive method using tandem mass spectrometry. J Allergy Clin Immunol. 2011; 127(6):1394-9. DOI: 10.1016/j.jaci.2011.03.040. View

15.

Tascanov M, Gonel A . How Do Contrast Agents Affect Cardiac Markers and Coagulation Tests? Experimental Study. Comb Chem High Throughput Screen. 2019; 22(5):355-360. DOI: 10.2174/1386207322666190603170438. View

16.

Tarini B, Christakis D, Welch H . State newborn screening in the tandem mass spectrometry era: more tests, more false-positive results. Pediatrics. 2006; 118(2):448-56. DOI: 10.1542/peds.2005-2026. View

17.

Kwon C, Farrell P . The magnitude and challenge of false-positive newborn screening test results. Arch Pediatr Adolesc Med. 2000; 154(7):714-8. DOI: 10.1001/archpedi.154.7.714. View

18.

Mohamadi-Nejad A, Moosavi-Movahedi A, Hakimelahi G, Sheibani N . Thermodynamic analysis of human serum albumin interactions with glucose: insights into the diabetic range of glucose concentration. Int J Biochem Cell Biol. 2002; 34(9):1115-24. DOI: 10.1016/s1357-2725(02)00031-6. View

19.

Shimamura Y, Maeda T, Ogawa Y, Nagai Y, Shinohara T, Takizawa H . Unusual manifestation of monoclonal gammopathy of undetermined significance: a false serum creatinine elevation. CEN Case Rep. 2019; 9(2):109-113. PMC: 7148409. DOI: 10.1007/s13730-019-00438-9. View

20.

Rees J, Deblonde G, An D, Ansoborlo C, Gauny S, Abergel R . Evaluating the potential of chelation therapy to prevent and treat gadolinium deposition from MRI contrast agents. Sci Rep. 2018; 8(1):4419. PMC: 5849765. DOI: 10.1038/s41598-018-22511-6. View