Infection Increases the Risk of Carotid Plaque Formation: Clinical Samples Combined with Bioinformatics Analysis

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Oct 9

PMID 37809782

Authors

Yi Chen

Ningning You

Chaoyu Yang

Jinshun Zhang

Affiliations

Soon will be listed here.

Abstract

Objective: Infection with () may increase atherosclerosis, which can lead to carotid plaque formation. Our study examined the relationship between infection and carotid plaque formation, and its underlying mechanisms.

Methods: A total of 36,470 people who underwent physical examination in Taizhou Hospital Health Examination Center from June 2017 to June 2022 were included in this study. All people participated in the urease test, neck ultrasound, blood pressure detection, anthropometric measurement and biochemical laboratory examination. In addition, the GSE27411 and GSE28829 datasets in the Gene Expression Omnibus (GEO) database were used to analyze the mechanism of infection and atherosclerosis progression.

Results: infection, sex, age, blood lipids, blood pressure, fasting blood glucose, glycated hemoglobin and body mass index were risk factors for carotid plaque formation. An independent risk factor was still evident in the multivariate logistic regression analysis, indicating infection. Furthermore, after weighted gene coexpression network analysis (WGCNA), we discovered 555 genes linked to both infection and the advancement of atherosclerosis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed a strong correlation between these genes and immunity, infection, and immune disorders. SsGSEA analysis showed that infection and atherosclerosis included changes in the immune microenvironment. Finally, three genes MS4A6A, ADAMDEC1 and AQP9 were identified to be involved in the formation of atherosclerosis after infection. Conclusion: Our research affirms that is a unique contributor to the formation of carotid plaque, examines the immune microenvironment associated with infection and advanced carotid atherosclerosis, and offers fresh perspectives on how infection leads to atherosclerosis.

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