Rapid Detection Methods for Foodborne Pathogens Based on Nucleic Acid Amplification: Recent Advances, Remaining Challenges, and Possible Opportunities

Overview

Journal Food Chem (Oxf)

Specialty Biochemistry

Date 2023 Sep 28

PMID 37767229

Authors

Nodali Ndraha

Hung-Yun Lin

Chen-Yow Wang

Hsin-I Hsiao

Han-Jia Lin

Affiliations

Soon will be listed here.

Abstract

This article presents a review of recent advancements in the utilization of NAA-based techniques for detecting foodborne pathogens in food products, focusing on studies conducted within the past five years. This review revealed that recent research efforts have primarily aimed at enhancing sensitivity and specificity by improving sample pre-treatment/preparation, DNA isolation, and readout methods. Isothermal-based amplification methods, such as LAMP, RPA, RAA, and RCA, have emerged as promising approaches, providing rapid results within one h and often demonstrating comparable or superior sensitivity to conventional or qPCR methods. However, the attention paid to specific pathogens varies, with spp., spp., , and receiving more focus than norovirus and other similar pathogens. NAA-based methods have the potential to significantly contribute to food safety and public health protection. However, further advancements are necessary to fully realize their benefits.

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