Colorimetric Detection of Norovirus by Helicase-dependent Amplification Method Based on Specific Primers Integrated with HRPzyme

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2022 Aug 5

PMID 35931785

Authors

Jeong-Eun Lee

Sol-A Kim

Hyun-Jin Park

Hyoyoung Mun

Kwang-Soo Ha

Won-Bo Shim

Affiliations

Soon will be listed here.

Abstract

Noroviruses (NoVs) are the most common causes of epidemic gastroenteritis, responsible for at least 50% of all gastroenteritis outbreaks worldwide and significant causes of foodborne illness. In the USA, approximately 21 million illnesses attributable to NoVs have annually occurred. Therefore, there is a great demand to develop a rapid, low-cost, and accurate detection method for NoVs. This study first reported colorimetric helicase-dependent amplification (HDA) methods based on specific primers integrated with HRPzyme for the rapid and sensitive detection of NoV GI and GII. The colorimetric HDA methods exhibited a detection limit of 10 copies mL of each NoV GI and GII and were confirmed to be specific to each NoV GI and GII. The period required to complete the HDA method was 2 h, including a step of RNA extraction and cDNA synthesis without expensive instruments such as a thermal cycler and detector. The cutoff value of the method for the oyster artificially inoculated with a known amount of NoV was all 10 copies g for NoV GI and GII. Therefore, the HDA method developed in this study can be useful tool for the on-site detection of NoVs in food samples.

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