Chitosan/Alginate Polymeric Nanoparticle-Loaded α-Mangostin: Characterization, Cytotoxicity, and In Vivo Evaluation Against Breast Cancer Cells

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2023 Sep 28

PMID 37765512

Authors

Muchtaridi Muchtaridi

Ade Irma Suryani

Nasrul Wathoni

Yedi Herdiana

Ahmed Fouad Abdelwahab Mohammed

Amirah Mohd Gazzali

Ronny Lesmana

I Made Joni

Affiliations

Soon will be listed here.

Abstract

α-mangostin (), a compound isolated from the mangosteen rind ( L.), has demonstrated promising anticancer activity. However, its low solubility and selectivity against cancer cells limit its efficacy. To address this issue, researchers have developed chitosan/alginate polymeric nanoparticles (NANO-AMCAL) to enhance the effectiveness of . In vitro studies have demonstrated that NANO-AMCAL is highly active against breast cancer cells. Therefore, an in vivo study was conducted to evaluate the efficacy of NANO-AMCAL in treating breast cancer in Wistar rats () and determine the effective dose. The rats were divided into seven treatment groups, including positive control, negative control, pure , and NANO-AMCAL 5 mg, 10 mg, and 20 mg. The rats were injected subcutaneously with a carcinogenic agent, 7,12-dimethylbenz(a)anthracene (DMBA) and were evaluated for weight and tumor volume every three days during treatment. Surgery was performed on day 14, and histopathological studies were carried out on breast and lung cancer tissues. The results showed that NANO-AMCAL significantly enhanced the anticancer activity of in treating breast cancer in Wistar rats. NANO-AMCAL containing 0.33 mg of had a healing effect three times better than 20 mg pure and was comparable to tamoxifen. The effective dose of NANO-AMCAL for anti-breast cancer treatment in Wistar rats was found to be 20 mg, which exhibited a good healing response, and the tumor volume continued to decrease up to 17.43% on the 14th day. Furthermore, histopathological tests showed tissue repair and no metastases. These findings suggest that NANO-AMCAL may be a promising therapeutic option for breast cancer treatment.

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