Study of Optimal Conditions to Grow Thai , Fruiting Test, Proximate and Their Alpha Glucosidase Inhibitory Activity

() has a worldwide distribution and has been widely used in traditional medicines. In this study, we report wild strains of that include two and one from northern Thailand. Optimal conditions for mycelium growth were ensured. The most favourable medium was potato sucrose agar for and oatmeal agar for and at 25 °C and 30 °C and pH 4-8. All types of cereal grains can be used to promote the growth of the mycelia of species. Fruiting tests were performed. All strains of produce fruiting bodies successfully in bag culture at 28 ± 1 °C with 75-85% relative humidity. Only produced fruiting bodies in field cultivation at the laboratory scale. In the first flush yields, the strain MFLUCC 22-0064 gave better production (the B.E was 152.35 ± 6.98 g). This study is the first to document the bag and field cultivation of wild Thai . species are revealed to contain high amounts of fiber (47.90-52.45% d.b.), protein (12.80-14.67% d.b.), fat (4.90-5.70% d.b.), and carbohydrates (3.16-4.02% d.b.). Additionally, and were preliminarily screened for biological activity for inhibition of alpha-glucosidase enzyme activity. The IC values of (MFLUCC 22-0066) was 105.97 ± 1.36 µg/mL and (MFLUCC 22-0064) was 126.94 ± 0.87 µg/mL. Both strains had better inhibition than acarbose (168.18 ± 0.89 µM). These results on wild strains of will be useful for further studies on the applications of . Later the species can be introduced to domestic markets for cultivation and medicinal use.