Molecular Characterization of Spp., , and in Dairy Goat Kids with Diarrhea in Partial Regions of Shaanxi Province, China

Overview

Journal Animals (Basel)

Date 2023 Sep 28

PMID 37760322

Authors

Xin Yang

Junwei Wang

Shuang Huang

Junke Song

Yingying Fan

Guanghui Zhao

Affiliations

Soon will be listed here.

Abstract

spp., , and are important diarrheal pathogens threatening the health of humans and various animals. Goats, especially pre-weaned goat kids, that carry these pathogens are important reservoirs related to human infection. In the present study, PCR-based sequencing techniques were applied to characterize spp., , and in 202 fecal samples of diarrheal kids for Guanzhong dairy goats from five locations in Shaanxi Province. The positive rates of spp., , and were 37.6% (76/202), 16.3% (33/202), 55.4% (112/202) and 78.7% (159/202) in these goat kids, respectively. Co-infection of two to four pathogens was found in 114 of 202 fecal samples. Significant differences ( < 0.001) in the positive rates of spp. and were found among locations and age groups. Furthermore, two species ( and ), two assemblages (E and A), nine genotypes (CHG3, CHG1, BEB6, CHG5, CHG2, SX1, CHG28, COS-II and CD6) and two pathotypes (EPEC and EHEC) were identified. As for , two (IIdA19G1 and IIdA19G2) and two (XXIIIa and XXIIIg) subtypes were recognized in samples positive for and , respectively. A phylogenetic analysis based on the ITS locus of indicated that all nine genotypes of identified in this study belonged to the group 2. Four virulence factors (, , and ) of EPEC and EHEC were found in strains. Collectively, this study explored the colonization frequency of spp., , and in diarrheal kids of Guanzhong dairy goats in Shaanxi Province and expanded our understanding of the genetic composition and zoonotic potential of these pathogens in goats.

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References

Yang X, Gong Q, Zhao B, Cai Y, Zhao Q . Prevalence of Infection in Sheep and Goat Flocks in China During 2010-2019: A Systematic Review and Meta-Analysis. Vector Borne Zoonotic Dis. 2021; 21(9):692-706. DOI: 10.1089/vbz.2020.2713. View

Stensvold C, Beser J, Axen C, Lebbad M . High applicability of a novel method for gp60-based subtyping of Cryptosporidium meleagridis. J Clin Microbiol. 2014; 52(7):2311-9. PMC: 4097674. DOI: 10.1128/JCM.00598-14. View

Shi K, Li M, Wang X, Li J, Karim M, Wang R . Molecular survey of Enterocytozoon bieneusi in sheep and goats in China. Parasit Vectors. 2016; 9:23. PMC: 5024852. DOI: 10.1186/s13071-016-1304-0. View

Zahedi A, Field D, Ryan U . Molecular typing of Giardia duodenalis in humans in Queensland - first report of Assemblage E. Parasitology. 2017; 144(9):1154-1161. DOI: 10.1017/S0031182017000439. View

Li N, Wang R, Cai M, Jiang W, Feng Y, Xiao L . Outbreak of cryptosporidiosis due to Cryptosporidium parvum subtype IIdA19G1 in neonatal calves on a dairy farm in China. Int J Parasitol. 2019; 49(7):569-577. PMC: 7089608. DOI: 10.1016/j.ijpara.2019.02.006. View

Li W, Feng Y, Santin M . Host Specificity of Enterocytozoon bieneusi and Public Health Implications. Trends Parasitol. 2019; 35(6):436-451. DOI: 10.1016/j.pt.2019.04.004. View

Sulaiman I, Fayer R, Lal A, Trout J, Schaefer 3rd F, Xiao L . Molecular characterization of microsporidia indicates that wild mammals Harbor host-adapted Enterocytozoon spp. as well as human-pathogenic Enterocytozoon bieneusi. Appl Environ Microbiol. 2003; 69(8):4495-501. PMC: 169096. DOI: 10.1128/AEM.69.8.4495-4501.2003. View

Yang F, Ma L, Gou J, Yao H, Ren M, Yang B . Seasonal distribution of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Tibetan sheep in Qinghai, China. Parasit Vectors. 2022; 15(1):394. PMC: 9615363. DOI: 10.1186/s13071-022-05442-0. View

Feng Y, Xiao L . Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev. 2011; 24(1):110-40. PMC: 3021202. DOI: 10.1128/CMR.00033-10. View

10.

Jian F, Liu A, Wang R, Zhang S, Qi M, Zhao W . Common occurrence of Cryptosporidium hominis in horses and donkeys. Infect Genet Evol. 2016; 43:261-6. DOI: 10.1016/j.meegid.2016.06.004. View

11.

Li W, Feng Y, Zhang L, Xiao L . Potential impacts of host specificity on zoonotic or interspecies transmission of Enterocytozoon bieneusi. Infect Genet Evol. 2019; 75:104033. DOI: 10.1016/j.meegid.2019.104033. View

12.

Berrilli F, DAlfonso R, Giangaspero A, Marangi M, Brandonisio O, Kabore Y . Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d'Ivoire: occurrence and evidence for environmental contamination. Trans R Soc Trop Med Hyg. 2012; 106(3):191-5. DOI: 10.1016/j.trstmh.2011.12.005. View

13.

Ryan U, Caccio S . Zoonotic potential of Giardia. Int J Parasitol. 2013; 43(12-13):943-56. DOI: 10.1016/j.ijpara.2013.06.001. View

14.

Xiao L, Feng Y . Molecular epidemiologic tools for waterborne pathogens spp. and . Food Waterborne Parasitol. 2020; 8-9:14-32. PMC: 7034008. DOI: 10.1016/j.fawpar.2017.09.002. View

15.

Ryan U, Feng Y, Fayer R, Xiao L . Taxonomy and molecular epidemiology of Cryptosporidium and Giardia - a 50 year perspective (1971-2021). Int J Parasitol. 2021; 51(13-14):1099-1119. DOI: 10.1016/j.ijpara.2021.08.007. View

16.

Li W, Liu X, Gu Y, Liu J, Luo J . Prevalence of Cryptosporidium, Giardia, Blastocystis, and trichomonads in domestic cats in East China. J Vet Med Sci. 2019; 81(6):890-896. PMC: 6612483. DOI: 10.1292/jvms.19-0111. View

17.

Zhang W, Zhang X, Wang R, Liu A, Shen Y, Ling H . Genetic characterizations of Giardia duodenalis in sheep and goats in Heilongjiang Province, China and possibility of zoonotic transmission. PLoS Negl Trop Dis. 2012; 6(9):e1826. PMC: 3447956. DOI: 10.1371/journal.pntd.0001826. View

18.

Li W, Wang K, Gu Y . Detection and Genotyping Study of Enterocytozoon bieneusi in Sheep and Goats in East-central China. Acta Parasitol. 2019; 64(1):44-50. DOI: 10.2478/s11686-018-00006-8. View

19.

Jiang W, Roellig D, Guo Y, Li N, Feng Y, Xiao L . Development of a Subtyping Tool for Zoonotic Pathogen . J Clin Microbiol. 2020; 59(3). PMC: 8106722. DOI: 10.1128/JCM.02474-20. View

20.

Karim M, Dong H, Yu F, Jian F, Zhang L, Wang R . Genetic diversity in Enterocytozoon bieneusi isolates from dogs and cats in China: host specificity and public health implications. J Clin Microbiol. 2014; 52(9):3297-302. PMC: 4313153. DOI: 10.1128/JCM.01352-14. View