Development of a Subtyping Tool for Zoonotic Pathogen

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2020 Dec 10

PMID 33298606

Citations 18

Authors

Wen Jiang

Dawn M Roellig

Yaqiong Guo

Na Li

Yaoyu Feng

Lihua Xiao

Affiliations

Soon will be listed here.

Abstract

is an important cause of cryptosporidiosis in canines and humans. Studies of the transmission characteristics of are currently hampered by the lack of suitable subtyping tools. In this study, we conducted a genomic survey of the pathogen and developed a subtyping tool targeting the partial 60-kDa glycoprotein gene (). Seventy-six isolates previously identified as were analyzed using the new subtyping tool. Amplicons of the expected size were obtained from 49 isolates, and phylogenetic analysis identified 10 subtypes clustered into five distinct groups (XXa to XXe). The largest group, XXa, contained 43 isolates from four subtypes that differed slightly from each other at the nucleotide level, while groups XXb to XXe contain one to three isolates each. The similar distributions of subtypes in humans and canines suggest that zoonotic transmission might play an important role in the epidemiology of In addition, suspected zoonotic transmission of between dogs and humans in a household was confirmed using the subtyping tool. The subtyping tool and data generated in this study might improve our understanding of the transmission of this zoonotic pathogen.

Citing Articles

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References

Lucio-Forster A, Griffiths J, Cama V, Xiao L, Bowman D . Minimal zoonotic risk of cryptosporidiosis from pet dogs and cats. Trends Parasitol. 2010; 26(4):174-9. DOI: 10.1016/j.pt.2010.01.004. View

Xu H, Jin Y, Wu W, Li P, Wang L, Li N . Genotypes of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in dogs and cats in Shanghai, China. Parasit Vectors. 2016; 9:121. PMC: 4774012. DOI: 10.1186/s13071-016-1409-5. View

Xiao L, Feng Y . Molecular epidemiologic tools for waterborne pathogens spp. and . Food Waterborne Parasitol. 2020; 8-9:14-32. PMC: 7034008. DOI: 10.1016/j.fawpar.2017.09.002. View

Boulter-Bitzer J, Lee H, Trevors J . Molecular targets for detection and immunotherapy in Cryptosporidium parvum. Biotechnol Adv. 2006; 25(1):13-44. DOI: 10.1016/j.biotechadv.2006.08.003. View

Xiao L . Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol. 2009; 124(1):80-9. DOI: 10.1016/j.exppara.2009.03.018. View

Checkley W, White Jr A, Jaganath D, Arrowood M, Chalmers R, Chen X . A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium. Lancet Infect Dis. 2014; 15(1):85-94. PMC: 4401121. DOI: 10.1016/S1473-3099(14)70772-8. View

Xiao L, Cama V, Cabrera L, Ortega Y, Pearson J, Gilman R . Possible transmission of Cryptosporidium canis among children and a dog in a household. J Clin Microbiol. 2007; 45(6):2014-6. PMC: 1933110. DOI: 10.1128/JCM.00503-07. View

Hijjawi N, Ng J, Yang R, Atoum M, Ryan U . Identification of rare and novel Cryptosporidium GP60 subtypes in human isolates from Jordan. Exp Parasitol. 2010; 125(2):161-4. DOI: 10.1016/j.exppara.2010.01.011. View

Li N, Xiao L, Alderisio K, Elwin K, Cebelinski E, Chalmers R . Subtyping Cryptosporidium ubiquitum,a zoonotic pathogen emerging in humans. Emerg Infect Dis. 2014; 20(2):217-24. PMC: 3901490. DOI: 10.3201/eid2002.121797. View

10.

Xiao L, Feng Y . Zoonotic cryptosporidiosis. FEMS Immunol Med Microbiol. 2008; 52(3):309-23. DOI: 10.1111/j.1574-695X.2008.00377.x. View

11.

Taghipour A, Olfatifar M, Bahadory S, Godfrey S, Abdoli A, Khatami A . The global prevalence of Cryptosporidium infection in dogs: A systematic review and meta-analysis. Vet Parasitol. 2020; 281:109093. DOI: 10.1016/j.vetpar.2020.109093. View

12.

Guo Y, Li N, Lysen C, Frace M, Tang K, Sammons S . Isolation and enrichment of Cryptosporidium DNA and verification of DNA purity for whole-genome sequencing. J Clin Microbiol. 2014; 53(2):641-7. PMC: 4298554. DOI: 10.1128/JCM.02962-14. View

13.

Cevallos A, Zhang X, Waldor M, Jaison S, Zhou X, Tzipori S . Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15. Infect Immun. 2000; 68(7):4108-16. PMC: 101706. DOI: 10.1128/IAI.68.7.4108-4116.2000. View

14.

Xiao L, Sulaiman I, Ryan U, Zhou L, Atwill E, Tischler M . Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Int J Parasitol. 2002; 32(14):1773-85. DOI: 10.1016/s0020-7519(02)00197-2. View

15.

Zhang X, Cong W, Ma J, Lou Z, Zheng W, Zhao Q . First report of Cryptosporidium canis in farmed Arctic foxes (Vulpes lagopus) in China. Parasit Vectors. 2016; 9:126. PMC: 4776383. DOI: 10.1186/s13071-016-1396-6. View

16.

Gonzalez-Diaz M, Urrea-Quezada A, Villegas-Gomez I, Durazo M, Garibay-Escobar A, Hernandez J . Cryptosporidium canis in Two Mexican Toddlers. Pediatr Infect Dis J. 2016; 35(11):1265-1266. DOI: 10.1097/INF.0000000000001287. View

17.

Stensvold C, Beser J, Axen C, Lebbad M . High applicability of a novel method for gp60-based subtyping of Cryptosporidium meleagridis. J Clin Microbiol. 2014; 52(7):2311-9. PMC: 4097674. DOI: 10.1128/JCM.00598-14. View

18.

Rojas-Lopez L, Elwin K, Chalmers R, Enemark H, Beser J, Troell K . Development of a gp60-subtyping method for Cryptosporidium felis. Parasit Vectors. 2020; 13(1):39. PMC: 6979280. DOI: 10.1186/s13071-020-3906-9. View

19.

Feng Y, Ryan U, Xiao L . Genetic Diversity and Population Structure of Cryptosporidium. Trends Parasitol. 2018; 34(11):997-1011. DOI: 10.1016/j.pt.2018.07.009. View

20.

Zhang S, Tao W, Liu C, Jiang Y, Wan Q, Li Q . First report of Cryptosporidium canis in foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) and identification of several novel subtype families for Cryptosporidium mink genotype in minks (Mustela vison) in China. Infect Genet Evol. 2016; 41:21-25. DOI: 10.1016/j.meegid.2016.03.016. View