Purification of Food Allergens from Their Natural Sources: Chromatographic Methods

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Sep 22

PMID 37737975

Authors

Sahil Gupta

Changqi Liu

Affiliations

Soon will be listed here.

Abstract

Isolation of food allergens from natural sources enables their characterization in a naturally occurring state. Low-pressure liquid chromatographic methods can be used to purify such allergens with high purity and yield. In this chapter, we describe the protocol for isolating a nut seed protein from its natural source. Interfering macromolecules such as fats and carbohydrates are removed followed by solubilization and extraction of nut seed proteins. The target protein is purified using a combination of various chromatographic techniques.

References

Sathe S, Liu C, Zaffran V . Food Allergy. Annu Rev Food Sci Technol. 2016; 7:191-220. DOI: 10.1146/annurev-food-041715-033308. View

Sutton B, Gould H . The human IgE network. Nature. 1993; 366(6454):421-8. DOI: 10.1038/366421a0. View

Gould H, Sutton B, Beavil A, Beavil R, McCloskey N, Coker H . The biology of IGE and the basis of allergic disease. Annu Rev Immunol. 2002; 21:579-628. DOI: 10.1146/annurev.immunol.21.120601.141103. View

Sathe S, Venkatachalam M, Sharma G, Kshirsagar H, Teuber S, Roux K . Solubilization and electrophoretic characterization of select edible nut seed proteins. J Agric Food Chem. 2009; 57(17):7846-56. DOI: 10.1021/jf9016338. View

Sathe S, Wolf W, Roux K, Teuber S, Venkatachalam M . Biochemical characterization of amandin, the major storage protein in almond (Prunus dulcis L.). J Agric Food Chem. 2002; 50(15):4333-41. DOI: 10.1021/jf020007v. View

Sharma G, Mundoma C, Seavy M, Roux K, Sathe S . Purification and biochemical characterization of Brazil nut (Bertholletia excelsa L.) seed storage proteins. J Agric Food Chem. 2010; 58(9):5714-23. DOI: 10.1021/jf9036326. View

Albillos S, Jin T, Howard A, Zhang Y, Kothary M, Fu T . Purification, crystallization and preliminary X-ray characterization of prunin-1, a major component of the almond (Prunus dulcis) allergen amandin. J Agric Food Chem. 2008; 56(13):5352-8. DOI: 10.1021/jf800529k. View

Reitsma M, Bastiaan-Net S, Sforza S, van der Valk J, van Gerth van Wijk R, Savelkoul H . Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3. J Agric Food Chem. 2016; 64(5):1191-201. DOI: 10.1021/acs.jafc.5b04401. View

Blanc F, Bernard H, Alessandri S, Bublin M, Paty E, Leung S . Update on optimized purification and characterization of natural milk allergens. Mol Nutr Food Res. 2008; 52 Suppl 2:S166-75. DOI: 10.1002/mnfr.200700283. View

10.

Yu C, Gao X, Lin H, Xu L, Ahmed I, Khan M . Purification, Characterization, and Three-Dimensional Structure Prediction of Paramyosin, a Novel Allergen of . J Agric Food Chem. 2020; 68(49):14632-14642. DOI: 10.1021/acs.jafc.0c04418. View

11.

Md A, Maeda M, Matsui T, Takasato Y, Ito K, Kimura Y . Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform. Glycoconj J. 2021; 38(1):67-76. DOI: 10.1007/s10719-020-09969-1. View

12.

Jiang B, Wang X, Wang L, Lv X, Li D, Liu C . Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean () Seeds. Polymers (Basel). 2019; 11(5). PMC: 6571848. DOI: 10.3390/polym11050785. View

13.

Kasera R, Singh A, Lavasa S, Nagendra K, Arora N . Purification and immunobiochemical characterization of a 31 kDa cross-reactive allergen from Phaseolus vulgaris (kidney bean). PLoS One. 2013; 8(5):e63063. PMC: 3650053. DOI: 10.1371/journal.pone.0063063. View

14.

Asturias J, Gomez-Bayon N, Arilla M, Martinez A, Palacios R, Sanchez-Gascon F . Molecular characterization of American cockroach tropomyosin (Periplaneta americana allergen 7), a cross-reactive allergen. J Immunol. 1999; 162(7):4342-8. View