Whole-genome Screens Reveal Regulators of Differentiation State and Context-dependent Migration in Human Neutrophils

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Sep 18

PMID 37723145

Authors

Nathan M Belliveau

Matthew J Footer

Emel Akdoan

Aaron P van Loon

Sean R Collins

Julie A Theriot

Affiliations

Soon will be listed here.

Abstract

Neutrophils are the most abundant leukocyte in humans and provide a critical early line of defense as part of our innate immune system. We perform a comprehensive, genome-wide assessment of the molecular factors critical to proliferation, differentiation, and cell migration in a neutrophil-like cell line. Through the development of multiple migration screen strategies, we specifically probe directed (chemotaxis), undirected (chemokinesis), and 3D amoeboid cell migration in these fast-moving cells. We identify a role for mTORC1 signaling in cell differentiation, which influences neutrophil abundance, survival, and migratory behavior. Across our individual migration screens, we identify genes involved in adhesion-dependent and adhesion-independent cell migration, protein trafficking, and regulation of the actomyosin cytoskeleton. This genome-wide screening strategy, therefore, provides an invaluable approach to the study of neutrophils and provides a resource that will inform future studies of cell migration in these and other rapidly migrating cells.

Citing Articles

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