Systematic Comparison of CRISPR/Cas9 and RNAi Screens for Essential Genes

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2016 May 10

PMID 27159373

Citations 216

Authors

David W Morgens

Richard M Deans

Amy Li

Michael C Bassik

Affiliations

Soon will be listed here.

Abstract

We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.

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