Establishment and Characterization of a Cell Line Derived from Bovine Tracheal Glands

Overview

Journal In Vitro Cell Dev Biol

Specialties Anatomy & Histology
Cell Biology

Date 1986 Oct 1

PMID 3771437

Citations 6

Authors

W E Finkbeiner

J A Nadel

C B Basbaum

Affiliations

Soon will be listed here.

Abstract

Bovine tracheal submucosal gland cells have been isolated by enzymatic digestion and serially propagated in tissue culture for more than 12 mo. (40 passages). The cells exhibit an epithelioid appearance at confluence and contain alcian blue (pH 2.5)/periodic acid-Schiff-positive material within cytoplasmic granules. By electron microscopy numerous osmiophilic secretory granules are seen. Maximal growth is observed when the cells are grown on human placental collagen-coated culture vessels in medium supplemented with 20% fetal bovine serum. Scintillation spectrometry revealed that radiolabeled precursor (35SO4) was incorporated into high molecular weight molecules and released from cells. Isoproterenol (10(-6) to 10(-3) M) stimulated the release of 35SO4. The maximal response to isoproterenol was completely inhibited by the beta-adrenergic antagonist propranolol. It is concluded that the cultured cells retain features of tracheal gland cells and may serve as a useful model of synthesis and secretion of macromolecules by tracheal gland cells.

Citing Articles

Roles of mast cell proteases in airways.

Nadel J Drugs. 1989; 37 Suppl 1:51-5; discussion 69-77.

PMID: 2667941 DOI: 10.2165/00003495-198900371-00011.

Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40.

Gruenert D, Basbaum C, Welsh M, Li M, Finkbeiner W, Nadel J Proc Natl Acad Sci U S A. 1988; 85(16):5951-5.

PMID: 2457904 PMC: 281883. DOI: 10.1073/pnas.85.16.5951.

Neutrophil elastase and cathepsin G stimulate secretion from cultured bovine airway gland serous cells.

Sommerhoff C, Nadel J, Basbaum C, Caughey G J Clin Invest. 1990; 85(3):682-9.

PMID: 2107207 PMC: 296483. DOI: 10.1172/JCI114492.

Characterization of epithelial cell cultures derived from human tracheal glands.

Chopra D, Shoemaker R, Taylor G, Mathieu P In Vitro Cell Dev Biol. 1991; 27(1):13-20.

PMID: 1707407 DOI: 10.1007/BF02630889.

Mucin-like glycoprotein secreted by cultured hamster tracheal epithelial cells. Biochemical and immunological characterization.

Wu R, Plopper C, Cheng P Biochem J. 1991; 277 ( Pt 3):713-8.

PMID: 1651700 PMC: 1151302. DOI: 10.1042/bj2770713.

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