CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Sep 9

PMID 37686249

Authors

Shuo Huang

Rui Dai

Zhiqi Zhang

Han Zhang

Meng Zhang

Zhangjun Li

Kangrui Zhao

Wenjun Xiong

Siyu Cheng

Buhua Wang

Yi Wan

Affiliations

Soon will be listed here.

Abstract

CRISPR/Cas systems have found widespread applications in gene editing due to their high accuracy, high programmability, ease of use, and affordability. Benefiting from the cleavage properties ( or ) of Cas enzymes, the scope of CRISPR/Cas systems has expanded beyond gene editing and they have been utilized in various fields, particularly in live-cell imaging and bioanalysis. In this review, we summarize some fundamental working mechanisms and concepts of the CRISPR/Cas systems, describe the recent advances and design principles of CRISPR/Cas mediated techniques employed in live-cell imaging and bioanalysis, highlight the main applications in the imaging and biosensing of a wide range of molecular targets, and discuss the challenges and prospects of CRISPR/Cas systems in live-cell imaging and biosensing. By illustrating the imaging and bio-sensing processes, we hope this review will guide the best use of the CRISPR/Cas in imaging and quantifying biological and clinical elements and inspire new ideas for better tool design in live-cell imaging and bioanalysis.

Citing Articles

CRISPR in clinical diagnostics: bridging the gap between research and practice.

Lafi Z, Ata T, Asha S Bioanalysis. 2025; 17(4):281-290.

PMID: 39902531 PMC: 11866644. DOI: 10.1080/17576180.2025.2459520.

A Comparison of Two Versions of the CRISPR-Sirius System for the Live-Cell Visualization of the Borders of Topologically Associating Domains.

Viushkov V, Lomov N, Rubtsov M Cells. 2024; 13(17.

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